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罗海婧, 张永清, 石艳华, 李鑫, 张耀文. 不同红小豆品种幼苗对干旱胁迫的生理响应[J]. 植物科学学报, 2014, 32(5): 493-501. DOI: 10.11913/PSJ.2095-0837.2014.50493
引用本文: 罗海婧, 张永清, 石艳华, 李鑫, 张耀文. 不同红小豆品种幼苗对干旱胁迫的生理响应[J]. 植物科学学报, 2014, 32(5): 493-501. DOI: 10.11913/PSJ.2095-0837.2014.50493
LUO Hai-Jing, ZHANG Yong-Qing, SHI Yan-Hua, LI Xin, ZHANG Yao-Wen. Effects of Drought Stress on the Physiological Characteristics of Different Adzuki Bean Varieties at the Seedling Stage[J]. Plant Science Journal, 2014, 32(5): 493-501. DOI: 10.11913/PSJ.2095-0837.2014.50493
Citation: LUO Hai-Jing, ZHANG Yong-Qing, SHI Yan-Hua, LI Xin, ZHANG Yao-Wen. Effects of Drought Stress on the Physiological Characteristics of Different Adzuki Bean Varieties at the Seedling Stage[J]. Plant Science Journal, 2014, 32(5): 493-501. DOI: 10.11913/PSJ.2095-0837.2014.50493

不同红小豆品种幼苗对干旱胁迫的生理响应

Effects of Drought Stress on the Physiological Characteristics of Different Adzuki Bean Varieties at the Seedling Stage

  • 摘要: 为筛选红小豆(Phaseolus angularis Linn.)幼苗抗旱性鉴定指标,采用盆栽控水方法,对红小豆3个品种‘保红947’、‘东北大红袍’、‘晋小豆5号’进行了苗期形态特征及根系生理生化特性的研究。结果表明:干旱胁迫抑制了红小豆3个品种植株的生长;重度干旱胁迫下,3个品种光系统Ⅱ(PSⅡ)的潜在活性(可变荧光/初始荧光, Fv/Fo)、最大光化学效率(可变荧光/最大荧光产量, Fv/Fm)、株高、叶面积、茎粗、根鲜重、根系活力、可溶性蛋白质含量等指标均随干旱胁迫强度的增加呈明显下降趋势,根冠比、根系脯氨酸(Pro)含量、可溶性糖含量、SOD活性、POD活性、MDA含量等指标随着干旱胁迫强度的增加呈明显上升趋势。综合各项生理指标,‘东北大红袍’在干旱胁迫下能保持相对较优的生理状态,抗旱性最强。通过主成分分析表明,株高、叶面积、茎粗、主根长、根鲜重、最大光化学效率、根系活力、可溶性糖含量、可溶性蛋白质含量、SOD活性、MDA含量等生理生化特性可作为鉴定红小豆苗期抗旱性的指标。

     

    Abstract: The aim of this study was to select the index of drought resistance in adzuki bean breeding. The effects of three different drought stresses on the physiomorphological characteristics and root associated physiological characteristics of the adzuki bean (‘Baohong 947’,‘Dongbeidahongpao’, and ‘Jinxiaodou 5’) during the seedling stage were studied in a pot experiment. Results showed that drought stress inhibited plant growth, with all indexes showing a certain regularity with increasing drought stress. Compared with the control, the indexes of the three adzuki bean varieties, especially that under severe drought stress, changed effectively. The potential activity of PSⅡ (variable fluorescence/minimal fluorescence, Fv/Fo), maximal photochemical efficiency (variable fluorescence/maximal fluorescence, Fv/Fm), plant height, leaf area, stem diameter, root fresh weight, root activity, and content of soluble protein all decreased, respectively. However, with the decrease in water, the root-shoot ratio, content of proline (Pro), content of soluble sugar, activity of SOD, activity of POD and content of MDA increased, respectively. In short, ‘Dongbeidahongpao’ maintained a relatively superior physiological state under drought stress, exhibiting higher drought resistance than the other two varieties. By principal component analysis, the plant height, leaf area, stem diameter, main root length, root fresh weight, maximal photochemical efficiency (Fv/Fm), root activity, content of soluble sugar, content of soluble protein, activity of SOD, and content of MDA can be used as indexes for identifying drought resistance of the adzuki bean.

     

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