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吕定豪, 刘安莉, 徐文芬, 何顺志. 中国蜘蛛抱蛋属植物DNA条形码研究[J]. 植物科学学报, 2018, 36(6): 784-789. DOI: 10.11913/PSJ.2095-0837.2018.60784
引用本文: 吕定豪, 刘安莉, 徐文芬, 何顺志. 中国蜘蛛抱蛋属植物DNA条形码研究[J]. 植物科学学报, 2018, 36(6): 784-789. DOI: 10.11913/PSJ.2095-0837.2018.60784
Lü Ding-Hao, Liu An-Li, Xu Wen-Fen, He Shun-Zhi. Study on the genus Aspidistra based on DNA barcoding[J]. Plant Science Journal, 2018, 36(6): 784-789. DOI: 10.11913/PSJ.2095-0837.2018.60784
Citation: Lü Ding-Hao, Liu An-Li, Xu Wen-Fen, He Shun-Zhi. Study on the genus Aspidistra based on DNA barcoding[J]. Plant Science Journal, 2018, 36(6): 784-789. DOI: 10.11913/PSJ.2095-0837.2018.60784

中国蜘蛛抱蛋属植物DNA条形码研究

Study on the genus Aspidistra based on DNA barcoding

  • 摘要: 利用植物DNA条形码候选序列matK、psbA-trnH、psbK-psbI和rbcL对蜘蛛抱蛋属(Aspidistra)植物的19种104批样品进行扩增和测序,并采用相似性搜索算法(BLAST)对各序列的鉴定效率进行评价,得出蜘蛛抱蛋属物种鉴定的最佳序列。结果显示,psbK-psbI的物种鉴定成功率为88.7%,在单一序列中成功率最高。通过多序列组合鉴定效率的比较,发现组合序列的鉴定成功率明显高于单一序列,其中matK+psbK-psbI)组合的鉴定成功率高达100%,基于该序列组合构建蜘蛛抱蛋属植物的系统发育树,结果显示同一物种的样品聚集度较好,多表现为单系。研究结果表明matK+psbK-psbI)序列组合可作为蜘蛛抱蛋植物种鉴定的最佳条形码序列。

     

    Abstract: In this study, plant DNA barcode (matK, psbA-trnH, psbK-psbI, and rbcL) candidate sequences were used for the PCR amplification and sequencing of 104 samples from 19 Aspidistra species. The amplification success rate, sequencing success rate, and intra- and inter-species genetic variation of each barcode sequence were investigated, with BLAST searching adopted to evaluate the identification efficiency of each sequence. Results showed that the identification success rate of psbK-psbI was 88.7%, which was the highest for a single sequence. The identification efficiency of multiple combinational sequences was also compared and found to be significantly higher than that of the single sequences, with the success rate of matK+(psbK-psbI) reaching 100%. In addition, the neighbor-joining tree constructed based on the matK+(psbK-psbI) combinational sequence showed that samples of the same species had better aggregation and were mostly monophyletic. Therefore, matK+(psbK-psbI) appears to be the best barcode sequence for interspecific identification of Aspidistra.

     

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