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孙清萍, 汪莉, 易平, 朱英国. 红莲型水稻不育系统花粉发育不同时期MADS-box基因家族的表达分析[J]. 植物科学学报, 2002, 20(5): 325-328.
引用本文: 孙清萍, 汪莉, 易平, 朱英国. 红莲型水稻不育系统花粉发育不同时期MADS-box基因家族的表达分析[J]. 植物科学学报, 2002, 20(5): 325-328.
SUN Qing-Ping, WANG Li, YI Ping, ZHU Ying-Guo. Expression Analysis of MADS-box Gene Family on Uni-nucleate and Bi-nucleate Stage Anthers on HL-CMS System[J]. Plant Science Journal, 2002, 20(5): 325-328.
Citation: SUN Qing-Ping, WANG Li, YI Ping, ZHU Ying-Guo. Expression Analysis of MADS-box Gene Family on Uni-nucleate and Bi-nucleate Stage Anthers on HL-CMS System[J]. Plant Science Journal, 2002, 20(5): 325-328.

红莲型水稻不育系统花粉发育不同时期MADS-box基因家族的表达分析

Expression Analysis of MADS-box Gene Family on Uni-nucleate and Bi-nucleate Stage Anthers on HL-CMS System

  • 摘要: 以红莲型水稻不育系、保持系、恢复系和杂种为材料,根据MADS-box的保守序列设计特异引物,并分别以OligodT12GC和OligodT12CG为锚定引物对花粉发育单核期和二核期的花药进行了差异展示分析。扩增共得到382条带,其中有组成型表达带22×8=176条。对差异带的统计分析表明:MADS-box基因家族参与了水稻CMS和育性恢复的核质互作。最后,对这些差异带与CMS和育性恢复之间的关系以及特异引物在研究复杂生物学现象中的应用进行了讨论。

     

    Abstract: This study analysised the differences of gene expression among the uni- and bi- nucleate stage anthers of the HL-CMS system with the method of differential display.382 binds were detected in all samples with the specific MADS-box primer and Oligo dT12GC and Oligo dT1212CG as anchor primers respectively.46.1% (22×8=176 binds) of which expressed constitutively among all the samples.The statistical analysis of the polymorphism binds showed that the MADS-box genes took an important role in the cytoplasm and nuclear interaction of CMSand fertility restoration in rice.At the last,this paper has discussed the relationship between the diversity binds and CMSand sterility restoration.The application of differential display using specific primer in complicated biological phenomena is also discussed.

     

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