Abstract: The effects of temperature on the biomass, astaxanthin yield and astaxanthin content of Haematococcus pluvialis cultured in imitation system of circular pond has been investigated. Results showed that the biomass, content of astaxanthin and astaxanthin yield of H. pluvialis all experienced a trend of increase-highest-decrease in the range of 15℃-25℃. The astaxanthin yield and astaxanthin content (DW) at 25℃ and 22℃ were significantly higher than the others and with no significant difference between them. The biomass and astaxanthin yield reached their minimum at 15℃, were 1.4 g, 2.49 mg/L, respectively, and reached their maximum at 25℃, were 2.68 g, 13.53 mg/L, respectively. While astaxanthin content reached its minimum at 15℃, was 0.54% and reached its maximum at 22℃, was 1.52%.