Abstract：PCR was used as a rapid alternative method to isolate allelic of wheat low molecular weight subunit(LMW-GS) genes.The primers specific to Glu-D3 locus were designed based on published LMW-GS gene sequence data.The PCR products were inserted into pUCm-T vector and transferred into E.coli DH5α.The size of PCR product was about 1287 bp,including partial promoter and whole coding sequences.The LMW-GS encoded by cloned gene in Xinjiang wheat,Rikeze,contained 8 Cys residuces,being grouped into TypeⅠ.This gene clone was applied in the gene function research by the method of gene engineering.
汪越胜;覃建兵;汪长东;何光源. 一个小麦低分子量谷蛋白基因的分离[J]. 植物科学学报, 2005, 23(6): 511-513.
WANG Yue-Sheng;QIN Jian-Bing;WANG Chang-Dong;HE Guang-Yuan. Isolation of a New Low-molecular-weight Glutenin Subunit Gene in Wheat. , 2005, 23(6): 511-513.
2005, Vol. 23 
