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汪越胜, 覃建兵, 汪长东, 何光源. 一个小麦低分子量谷蛋白基因的分离[J]. 植物科学学报, 2005, 23(6): 511-513.
引用本文: 汪越胜, 覃建兵, 汪长东, 何光源. 一个小麦低分子量谷蛋白基因的分离[J]. 植物科学学报, 2005, 23(6): 511-513.
WANG Yue-Sheng, QIN Jian-Bing, WANG Chang-Dong, HE Guang-Yuan. Isolation of a New Low-molecular-weight Glutenin Subunit Gene in Wheat[J]. Plant Science Journal, 2005, 23(6): 511-513.
Citation: WANG Yue-Sheng, QIN Jian-Bing, WANG Chang-Dong, HE Guang-Yuan. Isolation of a New Low-molecular-weight Glutenin Subunit Gene in Wheat[J]. Plant Science Journal, 2005, 23(6): 511-513.

一个小麦低分子量谷蛋白基因的分离

Isolation of a New Low-molecular-weight Glutenin Subunit Gene in Wheat

  • 摘要: 以新疆小麦日喀则基因组DNA为模板,采用Glu-D3位点特异引物进行PCR扩增。PCR产物插入pUCm-T载体,转化感受态大肠杆菌E.coliDH5α,获得阳性克隆。经测序发现该插入片段长度1287bp,包括了部分启动子序列和完整的编码序列。编码序列推导的蛋白质含有8个半胱氨酸残基,属于6类LMW-GS中的TypeⅠ类,为以后这类基因的功能研究提供了基因材料。

     

    Abstract: PCR was used as a rapid alternative method to isolate allelic of wheat low molecular weight subunit(LMW-GS) genes.The primers specific to Glu-D3 locus were designed based on published LMW-GS gene sequence data.The PCR products were inserted into pUCm-T vector and transferred into E.coli DH5α.The size of PCR product was about 1287 bp,including partial promoter and whole coding sequences.The LMW-GS encoded by cloned gene in Xinjiang wheat,Rikeze,contained 8 Cys residuces,being grouped into TypeⅠ.This gene clone was applied in the gene function research by the method of gene engineering.

     

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