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郝金花, 马晓丽, 王小花, 韩榕. He-Ne激光对UV-B辐射小麦幼苗抗氧化系统的影响[J]. 植物科学学报, 2007, 25(4): 350-355.
引用本文: 郝金花, 马晓丽, 王小花, 韩榕. He-Ne激光对UV-B辐射小麦幼苗抗氧化系统的影响[J]. 植物科学学报, 2007, 25(4): 350-355.
HAO Jin-Hua, MA Xiao-Li, WANG Xiao-Hua, HAN Rong. Effects of He-Ne Laser on Antioxidant Systems of Wheat Seedlings Exposed to Ultraviolet-B Radiation[J]. Plant Science Journal, 2007, 25(4): 350-355.
Citation: HAO Jin-Hua, MA Xiao-Li, WANG Xiao-Hua, HAN Rong. Effects of He-Ne Laser on Antioxidant Systems of Wheat Seedlings Exposed to Ultraviolet-B Radiation[J]. Plant Science Journal, 2007, 25(4): 350-355.

He-Ne激光对UV-B辐射小麦幼苗抗氧化系统的影响

Effects of He-Ne Laser on Antioxidant Systems of Wheat Seedlings Exposed to Ultraviolet-B Radiation

  • 摘要: 分别采用5 mW·mm-2He-Ne激光辐照、10.08 kJ·m-2·d-1的UV-B辐射及二者组合对‘晋麦8号’小麦幼苗进行处理,5 d后测定各处理幼苗叶片超氧阴离子(O2-)产生速率,丙二醛(MDA)、谷胱甘肽还原酶(GR)、抗坏血酸过氧化物酶(APX)、抗坏血酸(AsA)及类胡萝卜素(Car)的变化,分析He-Ne激光对增强UV-B辐射引起小麦损伤的修复效应。结果显示:He-Ne激光辐照可使UV-B辐射后小麦幼苗超氧阴离子的产生速率和MDA含量均减小,GR和APX活性升高,AsA和Car含量增加。表明超氧阴离子的产生速率、MDA、GR、APX、AsA和Car变化同小麦幼苗损伤修复的能力相关,一定剂量的He-Ne激光辐照可部分修复增强UV-B对小麦幼苗抗氧化系统的辐射损伤。

     

    Abstract: The seedlings of wheat(Jinmai 8)were exposed to He-Ne laser irradiation with 5 mW·mm-2 power density,enhanced UV-B radiation(10.08 kJ·m-2·d-1)and the combined treatment of He-Ne laser irradiation and enhanced UV-B radiation for 5 days.Changes on the production rate of superoxide radicals,activity of gutathione reductase(GR)and ascorbate peroxidase(APX);content of malondialdehyde(MDA),ascorbic acid(AsA)and carotenoid(Car)were measured to test the repair role of He-Ne laser irradiation.The results showed that He-Ne laser irradiation on the wheat seedlings resulted in decreasing of the production rate of superoxide radicals,the content of MDA,AsA and Car,the activity of GR and APX enhanced.It suggested that those changes in production rate of superoxide radicals,MDA,GR,APX,AsA and Car were responsible for the capacity of repair in the wheat.Therefore,the damage of wheat seedlings induced by enhanced UV-B radiation on antioxidant systems can be repaired partly by He-Ne laser irradiation.

     

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