石龙尾(<I>Limnophila sessiliflora</I> BI.)的组织培养与快速繁殖技术研究

顾福根; 孙丙耀; 韵宇飞; 练兆云

石龙尾(Limnophila sessiliflora BI.)的组织培养与快速繁殖技术研究

Studies on Tissue Culture and Rapid Propagation Technique of Limnophila sessiliflora BI.

摘要

摘要: 以石龙尾(Limnophila sessiliflora BI.)沉水枝带节茎段为外植体进行离体培养,研究外植体灭菌方法以及培养基中不同生长调节剂的浓度对其增殖、生根的影响。结果表明:以0.1%的HgCl2为灭菌剂,采用4 min+4 min、间歇4 h的间歇灭菌法,可以获得成活的无菌外植体15%;在1/2 MS+6-BA 2.0 mg·L^-1+NAA 0.1~0.2 mg·L^-1的增殖培养基上培养35 d,试管苗的增殖系数可达30.8以上;在1/2 MS+6-BA 0.3 mg·L^-1+NAA0.5 mg·L^-1的生根培养基上培养28 d后,可获得具3~5个侧枝的生根苗,平均每株生根数4.8条;炼苗后移植成活率100%。

Abstract: The in vitro culture of Limnophila sessiliflora was established by using the nodal stem segments from submerged branch as explants.The method of surface sterilization of explants,the effects of different plant growth regulators on the multiplication and rooting were investigated.The results showed that 15% sterile explants could be obtained by twice surface treatment with 0.1% HgCl2 for 4 min with an interval of 4 h.When cultured on a 1/2 MS multiplication medium supplemented with 2.0 mg·L^-1 of 6-BA and 0.1-0.2 mg·L^-1 of NAA for 35 d,the test-tube seedling exhibited a multiplication coefficient higher than 30.8.For optimal rooting,induction on a 1/2 MS rooting medium supplemented with 0.3 mg·L^-1 of 6-BA and 0.5 mg·L^-1 of NAA for 28 d could produce rooted plantlets with 3-5 lateral shoots,and each plantlet generated 4.8 roots on average.Rooted plantlets were acclimatized and transferred into outdoor aquatic environment with 100% survival ratio.

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