Abstract: Brassica napus×Brassica oleracea hybrids were obtained by embryo rescue tech-nology and identified by flow cytometry analysis,chromosome count of stigma cells,and pollen viability tests.Patterns of isozyme and protein of the real hybrids were studied by polyacrylamide gel electrophoresis.In addition,isozyme(SOD,EST,and COD) and protein patterns were compared with those of the parents,with obvious variations observed.Results also showed that the SOD and COD patterns of the hybrids were similar to Brassica oleracea,while the EST pattern was similar to the female parent.The protein pattern mainly showed parental complements,with new bonds also possessing their own characters.