Abstract: The diversity of plant species of the Yesanhe Nature Reserve was studied by field investigation and indoor analysis.The results showed there were 1106 species (including infraspecific taxon) of wild spermatophyte belonging to 533 genera and 150 families.The 150 families of wild spermatophyte were divided into 11 areal-types and 10 variety-types.Tropical elements were the dominant components, in which tropical families contributed 56.88% in total (excluding cosmopolitan families).The 533 genera of wild spermatophyte were divided into 14 area-types and 17 variety-types.Temperate elements were dominant components,in which temperate genera contributed 59.06% in total (excluding cosmopolitan genera).Compared with the flora of five neighboring areas,the flora of the Yesanhe Nature Reserve showed high similarity with Mulinzi Nature Reserve.The purpose of this study was to protect biological diversity and provide background information for the construction of Yesanhe Nature Reserve.