Abstract: The effects of different disinfectants on explants of Racomitrium japonicum were studied here. Suitable inoculation media were screened tentatively, and the effects of sucrose concentrations on growth of R. japonicum gametophytes were investigated. The results showed that a concentration of 0.02% HgCl₂ for 45-60 seconds was optimal for initiation cultures from fragments of gametophytes pre-immersed in 2% detergent for a period of time. The suitable inoculation medium for sterile explants was organic culture medium MS. Protonemal colonies and young gametophytes then grew well when 3% sucrose was added.