Abstract:
Geranylgeranyl pyrophosphate synthase(GGPPS) is an important target site for regulating diterpenoid biosynthesis in plant cells. Now,a novel
geranylgeranyl pyrophosphate synthase gene (
SmGGPPS3) was cloned from the medicinal plant
Salvia miltiorrhiza. The full-length
SmGGPPS3 consists of 2908 nucleotides,including a 931-bp intron and one 960-bp ORF encoding region. The deduced protein of
SmGGPPS3 showed more than 67% similarity with GGPPSs from
Ricinus communis,rubber and
Arabidopsis.Quantitative RT-PCR analysis revealed that
SmGGPPS3 was significantly expressed in different development stages and different tissues examined,and could be induced by methyl jasmonate(MeJA) and pathogens.Furthermore,the genetic complementation expression of
SmGGPPS3 in
Escherichia coli revealed that
SmGGPPS3 encoded a functional GGPP synthase.