Abstract: Based on 1500 bryophyte sample collections and literature review, 206 species in 65 genera and 34 families of bryophytes from nine districts of Chongqing city are reported in the present paper. Twenty-five species belonging to 14 genera and 13 families were liverworts, and 181 species belonging to 51 genera and 21 families were mosses. Eighty species were recorded in Chongqing city for the first time. The checklist is provided below. Families with more than nine species included Pottiaceae, Brachytheciaceae, Bryaceae, Hypnaceae, Entodontaceae and Thuidiaceae, accounting for 62.14% of total species. Genera with more than five species included Brachythecium, Bryum, Entodon, Eurhynchium, Trichostomum, Fissidens, Hyophila and Hypnum, accounting for 41.26% of total species. Among the floristic types, North Temperate and East Asia were dominant, accounting for 32.75% and 30.41%, respectively, followed by tropical and endemic types. Compared with four adjacent areas, bryoflora in the nine districts of Chongqing had the highest similarity index with the urban area of Hangzhou at both the genera and species levels, but lower similarity indices with the three closer areas. This could be attributed to the same latitude and man-made urban habitats in the nine districts of Chongqing and the urban area of Hangzhou. The present study provides more information about bryophyte flora of Chongqing city, which will be helpful for compiling a bryophyte checklist for the whole city.