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董超, 杨云强, 孙旭东, 李雄, 杨时海, 黄蓉, 杨永平. 丝颖针茅ScTIP1;1基因的克隆及对非生物胁迫的应答[J]. 植物科学学报, 2016, 34(1): 99-108. DOI: 10.11913/PSJ.2095-0837.2016.10099
引用本文: 董超, 杨云强, 孙旭东, 李雄, 杨时海, 黄蓉, 杨永平. 丝颖针茅ScTIP1;1基因的克隆及对非生物胁迫的应答[J]. 植物科学学报, 2016, 34(1): 99-108. DOI: 10.11913/PSJ.2095-0837.2016.10099
DONG Chao, YANG Yun-Qiang, SUN Xu-Dong, LI Xiong, YANG Shi-Hai, HUANG Rong, YANG Yong-Ping. Molecular Cloning and Expression of ScTIP1;1 in Stipa capillacea under Abiotic Stress[J]. Plant Science Journal, 2016, 34(1): 99-108. DOI: 10.11913/PSJ.2095-0837.2016.10099
Citation: DONG Chao, YANG Yun-Qiang, SUN Xu-Dong, LI Xiong, YANG Shi-Hai, HUANG Rong, YANG Yong-Ping. Molecular Cloning and Expression of ScTIP1;1 in Stipa capillacea under Abiotic Stress[J]. Plant Science Journal, 2016, 34(1): 99-108. DOI: 10.11913/PSJ.2095-0837.2016.10099

丝颖针茅ScTIP1;1基因的克隆及对非生物胁迫的应答

Molecular Cloning and Expression of ScTIP1;1 in Stipa capillacea under Abiotic Stress

  • 摘要: 利用丝颖针茅(Stipa capillacea Keng)一条EST序列并结合cDNA末端快速扩增(RACE)技术,克隆了丝颖针茅的一个液泡膜内在蛋白(tonoplast intrinsic proteins,TIPs)基因ScTIP1;1的全长编码区序列.该基因开放阅读框长度为753 bp,编码250个氨基酸,其蛋白质分子量为25.8 kD,理论等电点为6.16;序列比对及系统进化分析表明,ScTIP1;1和拟南芥(Arabidopsis thaliana)AtTIP1;1蛋白的亲缘关系较近;亚细胞定位结果显示,该蛋白位于液泡膜上;实时荧光qRT-PCR检测表明,盐、干旱及低温胁迫可诱导ScTIP1;1基因的表达,且低温处理后基因的表达量变化最为明显.本研究结果为理解丝颖针茅的生态适应性提供了理论依据.

     

    Abstract: Based on EST sequence and RACE experiments, the full-length coding sequence of a tonoplast intrinsic protein (TIP) gene termed ScTIP1;1 was cloned from Stipa capillacea. Sequence analysis showed that the opening reading frame (ORF) of ScTIP1;1 was 753 bp, encoding a 250 amino acid peptide with a protein molecular weight of 25.8 kD and isoelectric point of 6.16. Phylogenetic analysis and sequence alignment results indicated that ScTIP1;1 had a close relationship with AtTIP1;1 of Arabidopsis thaliana. Subcellular localization analysis indicated that ScTIP1;1 was localized in the tonoplast membrane. The expression level of ScTIP1;1 was induced by salt, drought and especially low temperature in the leaves of S. capillacea. These results provide new insight into understanding the ecological adaptations of S.capillacea.

     

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