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徐乐乐, 张艳红, 周秀秀, 刁英, 胡中立, 靳素荣. 芒草中总黄酮的提取工艺研究及其挥发油成分的鉴定[J]. 植物科学学报, 2016, 34(4): 622-629. DOI: 10.11913/PSJ.2095-0837.2016.40622
引用本文: 徐乐乐, 张艳红, 周秀秀, 刁英, 胡中立, 靳素荣. 芒草中总黄酮的提取工艺研究及其挥发油成分的鉴定[J]. 植物科学学报, 2016, 34(4): 622-629. DOI: 10.11913/PSJ.2095-0837.2016.40622
XU Le-Le, ZHANG Yan-Hong, ZHOU Xiu-Xiu, Diao Ying, HU Zhong-Li, JIN Su-Rong. Optimal Total Flavonoids Extraction and Identification of Volatile Oil Components in Miscanthus sinensis[J]. Plant Science Journal, 2016, 34(4): 622-629. DOI: 10.11913/PSJ.2095-0837.2016.40622
Citation: XU Le-Le, ZHANG Yan-Hong, ZHOU Xiu-Xiu, Diao Ying, HU Zhong-Li, JIN Su-Rong. Optimal Total Flavonoids Extraction and Identification of Volatile Oil Components in Miscanthus sinensis[J]. Plant Science Journal, 2016, 34(4): 622-629. DOI: 10.11913/PSJ.2095-0837.2016.40622

芒草中总黄酮的提取工艺研究及其挥发油成分的鉴定

Optimal Total Flavonoids Extraction and Identification of Volatile Oil Components in Miscanthus sinensis

  • 摘要: 以黄酮提取率为指标,采用超声辅助法研究了芒草中黄酮类化合物的提取工艺。最佳提取条件为:乙醇浓度70%、料液比1:30、提取温度70℃、提取时间40 min、提取级数3次,在此条件下测得南荻中总黄酮含量为7.24 mg/g,并在此条件下测定了芒草10个品系中总黄酮含量为4.16~7.84 mg/g,且在不同品系间的差异均达到极显著水平。采用气质联用技术鉴定奇岗六倍体、‘GMG121’、芒杂中的挥发油成分,从中共鉴定出28种成分。本研究对芒属植物的遗传育种以及开发和综合利用具有重要意义。

     

    Abstract: An ultrasonic assisted method was used to study the optimum extraction of flavonoids in Miscanthus. The determined optimal extraction conditions of flavonoids were as follows:70% ethanol concentration, 1:30 ratio of raw material to liquid, 70℃ extraction temperature, 40 min extraction time, and three extract series, respectively. Under these conditions, total flavonoid content of M.lutarioriparius was 7.24 mg/g. The experimental yields of flavonoids from various kinds of M.sinensis were significantly different, ranging from 4.16 mg/g to 7.84 mg/g. The volatile oil components in Miscanthus x giganteus (hexaploid),‘GMG121’, and M.sinensis hybrid were determined by GC/MS, with 28 different components identified. This study provides useful information on genetic breeding, which has great significance for the development and utilization of M.sinensis.

     

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