Construction of a Plant Transgenic Expression Vector of Peanut
WANG Jing1,2, YAN Hai-Yan1, KANG Qiang-Sheng1
1. Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan 430074, China;2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
Abstract：The promoter of the gene encoding the major peanut allergy protein Ara h 1 has been cloned by the polymerase chain reaction technique(PCR) with a pair of special primers.The promoter we have got is 1957 bp.Basically,it is consistent with the sequence published.By replacing the 35S promoter in pBIN 35S-mGFP4 vector with this promoter,we have constructed a plant transgenic expression vector of peanut named pGA1.It contains seed specifically expressed elements,enhancers and some elements regulated by transcription factors.Thus the heterologous gene can be transferred to peanut and expresses in it easily.The research lays a foundation for establishing the peanut bioreactor.
王静;严海燕;康强胜. 一种花生转基因表达载体的构建[J]. 植物科学学报, 2005, 23(6): 514-518.
WANG Jing;YAN Hai-Yan;KANG Qiang-Sheng. Construction of a Plant Transgenic Expression Vector of Peanut. , 2005, 23(6): 514-518.
2005, Vol. 23 
