Abstract: Random amplified polymorphic DNA(RAPD) was applied to germplasm accessions in 29 flower-lotus cultivars(including both Nelumbo nucifera Gaertn.and N.lutea Pers.).A total of 207 bands were obtained from 17 selected primers,of which 122 were polymorphic.The results also showed that:(1) Two main groups were gained:those cultivars,with bigger flower,were mainly clustered in one group,and the other contained mass small-flower types.(2) No obvious difference was observed between N.lutea and all other cultivars(N.nucifera) from their DNA.Furthermore,it seemed that N.lutea was close to small-flower cultivars of N.nucifera.