Factors Affecting in vitro Shoot Elongation of Pepper
ZHANG Yu-Yang1,3, TANG Yin-Zhu1,4, YE Zhi-Biao1,3, ZHANG Chan-Juan3, LI Han-Xia1,2
1. National Center for Vegetable Improvement(Central China), Huazhong Agricultural University, Wuhan 430070, China;2. Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China;3. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China;4. Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan 430074, China
Abstract：A detailed study was carried out to investigate key factors affecting shoot elongation of pepper.Using the cotyledons of pepper as explants,the optimal phytohormone combination of BA+IAA for bud induction was screened out to investigate the effect of GA3,bud-inducting time,organic constituents in media,different phytohormone combinations and cultivars on shoot elongation.The results were as follows:Seven cultivars showed ability differences for bud induction,and BA 3.0 mg·L-1+IAA 0.5 mg·L-1 could give the highest response.The shoot elongation frequency increased with GA3 concentration,with the optimal concentration of 1.0-2.0 mg·L-1.The buds subjected to 21-day induction showed higher elongation efficiency than that of 14-day induction.B5 vitamins were more efficient than MS vitamins for shoot elongation.The phytohormone combination of Zeatin+GA3 exhibited higher elongation efficiency than combinations of BA+IAA+GA3 or BA+PAA+GA3.Elongation ability varied with pepper cultivars,with Chufeng and Sujiao No.5 having the best elongation ability.Compared with IAA and NAA,IBA gave better rooting effect.
2007, Vol. 25 
