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林荔辉, 汪斌, 陶爱芬, 吴建梅, 孙志强, 方平平, 祁建民. 用ISSR标记分析红麻种质资源的遗传多样性[J]. 植物科学学报, 2008, 26(3): 240-244.
引用本文: 林荔辉, 汪斌, 陶爱芬, 吴建梅, 孙志强, 方平平, 祁建民. 用ISSR标记分析红麻种质资源的遗传多样性[J]. 植物科学学报, 2008, 26(3): 240-244.
LIN Li-Hui, WANG Bin, TAO Ai-Fen, WU Jian-Mei, SUN Zhi-Qiang, FANG Ping-Ping, QI Jian-Min. The Analysis of Genetic Diversity of Kenaf Germplasm Based on Inter-simple Sequence Repeats[J]. Plant Science Journal, 2008, 26(3): 240-244.
Citation: LIN Li-Hui, WANG Bin, TAO Ai-Fen, WU Jian-Mei, SUN Zhi-Qiang, FANG Ping-Ping, QI Jian-Min. The Analysis of Genetic Diversity of Kenaf Germplasm Based on Inter-simple Sequence Repeats[J]. Plant Science Journal, 2008, 26(3): 240-244.

用ISSR标记分析红麻种质资源的遗传多样性

The Analysis of Genetic Diversity of Kenaf Germplasm Based on Inter-simple Sequence Repeats

  • 摘要: 为明确红麻种质资源的遗传多样性和亲缘关系,以20个国家的32份红麻(Hibiscus cannabinus L.)种质资源为材料,从90个ISSR引物中筛选出22个多态性引物对供试材料进行PCR扩增,最终选用条带最清晰、多态性明显的13个引物的扩增数据进行统计分析。结果表明:(1)13条ISSR多态性引物共扩增出谱带总数59条,多态性条带总数为47条,多态性条带比率达79.66%,其遗传多样性较丰富;(2)在切割线L1取值为0.73时,可将供试材料分为一个由22份栽培品种构成的L1-1大类群,以及由8份野生材料和2份古老地方品种构成的3个独立的个类和3个不同小类群。(3)当切割线L2取值为0.77时,又可将第1个大类群L1-1中22份栽培品种根据其亲缘关系,重新划分为I1、I2、I3、I44个不同的亚类群,各亚类群表现出明显的地域性特点。(4)聚类结果显示,红麻多数栽培品种之间的遗传差异较小,亲缘关系较近,其遗传多样性比野生材料明显狭窄。

     

    Abstract: In order to probe the relationship and genetic diversity of the kenaf(Hibiscus cannabinus)germplasm,32 kenaf varieties from 20 countries were studied,and 22 primers screened from 90 ISSR primers were used to amplify the DNA,from which 13 primers with polymorphic and clear bands were analysized.The results were as follows:(1) 59 bands were amplified based on 13 primers,among which 47 bands were polymorphic,and the percentage of polymorphic bands was 79.66%,which indicated the genetic diversity was correspondingly rich.(2)The 32 kenaf accessions could be classified into two groups at the level of D=0.73:one large group named L1-1 constituted of 22 cultivars,there antique kinds and there subgroup including 8 wild varieties and 2 antique local species.(3)The 22 cultivars in the first group L1-1 could be divided into 4 subclasses(I1,I2,I3,I4) at the level of D=0.77,which had visible regional character.(4)The cluster result indicated that most of the cultivars had small genetic diversity and relationship.It was obvious that the basis of the inheritance of kenaf cultivars were comparatively narrower than the wild species.

     

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