葡萄果实生长发育过程中白藜芦醇及其糖苷的HPLC测定

HPLC Analysis of Resveratrol and Piceid during the Development of Grape Berries

摘要: 利用高效液相色谱(HPLC)梯度洗脱方法,结合C₁₈固相萃取技术,检测了葡萄果实生长发育过程中顺反式白藜芦醇及其糖苷的动态变化。结果显示,适于酿造红葡萄酒的蛇龙珠和酿造白葡萄酒的白羽品种的白藜芦醇及其糖苷含量的变化规律大不相同。葡萄果实生长发育期间,蛇龙珠果实内的白藜芦醇及其糖苷含量较高,而白羽果实中白藜芦醇含量很低,其糖苷含量则持续下降。

Abstract: Resveratrol(Res) and piceid(PD) in grape berries were determined by using high performance liquid chromatography(HPLC),combining with the C₁₈ solid extraction.The result showed that the contents of Res in Carbernet Gernischet and Rkatsiteli existed obvious difference during the development of grapes berries.The contents of Res and PD were high in Carbernet Gernischet grapes berries,while the content of Res was low and the content PD kept decreasing during the development of Rkatsiteli grapes berries.