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杜延茹, 贾媛, 李洪泉. 盐生杜氏藻质膜ATPase及其对高渗震动的反应[J]. 植物科学学报, 1999, 17(1): 10-14.
引用本文: 杜延茹, 贾媛, 李洪泉. 盐生杜氏藻质膜ATPase及其对高渗震动的反应[J]. 植物科学学报, 1999, 17(1): 10-14.
Du Yanru, Jia Yuan, Li Hongquan. PLASMALEMMA ATPase OF DUNALIELLA SALINA AND ITS RELATION TO HYPEROSMOTIC SHOCK[J]. Plant Science Journal, 1999, 17(1): 10-14.
Citation: Du Yanru, Jia Yuan, Li Hongquan. PLASMALEMMA ATPase OF DUNALIELLA SALINA AND ITS RELATION TO HYPEROSMOTIC SHOCK[J]. Plant Science Journal, 1999, 17(1): 10-14.

盐生杜氏藻质膜ATPase及其对高渗震动的反应

PLASMALEMMA ATPase OF DUNALIELLA SALINA AND ITS RELATION TO HYPEROSMOTIC SHOCK

  • 摘要: 用水溶性多聚物(dextranT500/PEG3350)两相法制备盐生杜氏藻细胞质膜,经检测质膜纯度较高,原位膜约占78%。质膜ATPase的动力学常数Km和Vmax分别为0.58mmolL和43.59μmolPi/(mgprotein·h);最适pH值是7.5。质膜ATPase的活性随MgCl2和CaCl2浓度的升高而增加,但较高浓度的MgCl2和CaCl2有轻微的抑制作用;KCl促进质膜ATPase的活性,在100mmolL时达到最大,高于100mmolL时抑制效应显著。钒酸钠、DES、DCCD和NEM明显地抑制质膜ATPase的活性;而NaN3、NaNO3、NaMoO4和KCN对质膜ATPase的活性影响不大。高渗震动刺激了质膜ATPase的活性。

     

    Abstract: A fraction enriched in plasma membrane from Dunaliella salina was obtained by apueous two-phase partition system (dextran T500/polyethylene glycol 3350),with high quality and purity.The Km of plasmalemma ATPase was found to be 0.58 mmol/L,its Vmax was 43.59 μmol Pi/(mg protein·h) and the optimum pH 7.5.Enzyme activity was stimulated by the increase of concentration of MgCl2,CaCl2 and KCl,but much higher concentrations had a little inhibitory effect.The plasmalemma ATPase exhibited great sensitivities to vanadate,dicyeclohexyl carbodiimide (DCCD),SH regent N ethylmaleimide (NEM),especially to diethylstibestro (DES),whereas it was insensitive to azide,nitrate,molybdate and cyanide.The ATPase activity was notably increased following hyperosmotic shock,and its possible role in osmoregulation was discussed.

     

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