Abstract: The paper introduces a simple, efficient and fast bio analytic method for isozyme analysis: ultra thin slab IEF PAGEsystem.In comparison with other commercial products (such as BIO RAD model 111 Mini IEFcell, etc.), the system has many advantages: (1)more flexibility in applications;(2)wider range of applications;(3)higher resolution separations;(4)larger capacity of running samples;(5)shorter time for electrophoresis;(6)lower cost;(7)suitable for various enzyme systems;and (8)no need for gel drying.