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王鸣刚, 罗茂春, 张向红, 沈明山, 陈睦传. 低能碳、氮离子对甜叶菊萌发率、生长量及过氧化物酶的影响[J]. 植物科学学报, 2001, 19(2): 143-148.
引用本文: 王鸣刚, 罗茂春, 张向红, 沈明山, 陈睦传. 低能碳、氮离子对甜叶菊萌发率、生长量及过氧化物酶的影响[J]. 植物科学学报, 2001, 19(2): 143-148.
WANG Ming-Gang, LUO Mao-Chun, ZHANG Xiang-Hong, SHEN Ming-Shan, CHEN Mu-Chuan. Effects of Low Energy Carbon Ion and Nitrogen Ion Implantation on Germinant Rate, Growth Weight and Peroxidase of Stevia rebaudiana Bertoni[J]. Plant Science Journal, 2001, 19(2): 143-148.
Citation: WANG Ming-Gang, LUO Mao-Chun, ZHANG Xiang-Hong, SHEN Ming-Shan, CHEN Mu-Chuan. Effects of Low Energy Carbon Ion and Nitrogen Ion Implantation on Germinant Rate, Growth Weight and Peroxidase of Stevia rebaudiana Bertoni[J]. Plant Science Journal, 2001, 19(2): 143-148.

低能碳、氮离子对甜叶菊萌发率、生长量及过氧化物酶的影响

Effects of Low Energy Carbon Ion and Nitrogen Ion Implantation on Germinant Rate, Growth Weight and Peroxidase of Stevia rebaudiana Bertoni

  • 摘要: 以经过碳、氮(75×1014keV/cm2)离子注入处理后的甜叶菊(Steviarebaudiana Bertoni)种子为材料,研究低能离子注入对甜叶菊萌发率、生长量及过氧化物同工酶的影响。结果表明:(1)萌发期处理组的萌发率低于对照,萌发延迟,幼苗成活率不及对照组;萌发后期处理组的相对生长量、过氧化物酶活性高于对照组。(2)幼苗移至实验地种植后,处理组幼苗长势明显优于未处理组,处理组60d蕾株比明显高于对照组。(3)过氧化物酶同工酶谱分析发现,对照组具有完整6条基本带的植株数为12,具有完整B1、B2基本带的植株数为20;C+处理组分别为5和11,N+处理组分别为5和6。统计发现低能离子的注入影响最大的是同工酶谱B1、B2带。N+处理组生物学负效应大于C+处理组。

     

    Abstract: The paper reports the effects of low energy Carbon and Nitrogen ion (75×1014keV/cm2 )implantation into S. rebaudiana seeds.on germinant rate, relative growth weight and peroxidase of S. rebaudiana. The results showed: (1)The seed germinant rates were lower and seeding servival rates and deve lopment speeds were slower in the treated groups than those in the control in the initial period of seed germinating;but in the late period of seed germinating the relative growth weight and peroxidase activity were higher in treated groups than those in the control.(2)When seedings were transplanted into experimental field the growth and development were speeded up in treated groups than that in the control.The rate of flower bud plants were higher in treated groups than that in the control. (3)The isozymogram of peroxidase by PAGEshowed that there were 12 plants which had 1~6 basic bands and 20 plants which had B1,B2 bands in the control,but only there were 5 plants and 11 plants, respectively, in C+ treated groups,and there were 5 plants and 6 plants, respectively, in N+ treated groups. The result also showed there were more negative effects by ion implantation into S. rebaudiana in N+ treated groups than those in C+ treated groups in the periods of seed germinating and plant growth.

     

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