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欧阳叶新, 施定基, 胡鸿钧, 梁承邺. 几种因素诱导鱼腥藻7120短藻丝体的形成[J]. 植物科学学报, 2001, 19(5): 403-408.
引用本文: 欧阳叶新, 施定基, 胡鸿钧, 梁承邺. 几种因素诱导鱼腥藻7120短藻丝体的形成[J]. 植物科学学报, 2001, 19(5): 403-408.
OUYANG Ye-Xin, SHI Ding-Ji, HU Hong-Jun, LIANG Cheng-Ye. Formation of Short Filaments of Anabaena sp. PCC7120 Induced by Several Factors[J]. Plant Science Journal, 2001, 19(5): 403-408.
Citation: OUYANG Ye-Xin, SHI Ding-Ji, HU Hong-Jun, LIANG Cheng-Ye. Formation of Short Filaments of Anabaena sp. PCC7120 Induced by Several Factors[J]. Plant Science Journal, 2001, 19(5): 403-408.

几种因素诱导鱼腥藻7120短藻丝体的形成

Formation of Short Filaments of Anabaena sp. PCC7120 Induced by Several Factors

  • 摘要: 丝状蓝藻鱼腥藻7120(.PCC7120)中可成功表达外源基因,但其转化和表达效率不高,改变细胞的生理状态可能会影响外源基因的转化和表达效率。将鱼腥藻7120营养藻丝体通过几种因素诱导形成短藻丝体(具有25个左右细胞),并对其光合活性进行了测定。结果表明:红光和高温对鱼腥藻7120短藻丝体较为有效,且红光诱导在48h时,短藻丝体细胞数占总细胞数的比例达到85%;DCMU单独诱导效果不明显,适当浓度的DCMU+红光诱导时诱导效率略有增加;高温以45℃诱导12h比例最高,约达87%;高温45℃诱导时,对数生长后期的鱼腥藻7120较易形成短藻丝体。光合活性测定结果显示,诱导形成的短藻丝体光合放氧速率比正常营养藻丝体的低,这种具有光合放氧能力的短藻丝体显示出作为表达外源基因受体的可能性。

     

    Abstract: Foreign genes can be successfully expressed in the filamentous blue-green algae A nabaena sp. PCC7120 (A. 7120) at low levels. Changes in the physiological conditions of A. 7120 may affect expression of heterogenous genes in A. 7120. We described here that several stress factors induced the formation of short filaments (ca. 25 cells) from vegetative filaments of A. 7120. The data showed that red light and high temperature are efficient to induce the formation of short filaments; and after 48 h of red light induction, the ratio of the cell numbers of short filaments (CNSF) to the total cell numbers of vegetative filaments (CNT) is up to 85%. Meanwhile, DCMU is not efficient to induce the formation of short filaments, but the ratio of CNSF to CNT induced by red light supplemented with adequate concentration of DCMU in medium BG-11 can be increased slightly. High temperature at 45℃ treatment after 12 h can generate the highest ratio of CNSF to CNT about 87%. Furthermore, the exponential mid- or telo-phase growth of A. 7120 vegetative filamentous cells at 45℃ can easily form the short filaments. The photosynthetic analysis showed that the photosynthetic rates of oxygen evolution in short filaments are lower than those in normal vegetative filaments. It showed potential use of these A. 7120 short filaments with photosynthetic activities as recipient cells for expressing foreign genes.

     

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