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胡丰林, 陆瑞利. 尼日利亚螫毛果(Cnestis ferruginca)中一种具有清除自由基的活性化合物的分离鉴定[J]. 植物科学学报, 2002, 20(4): 311-314.
引用本文: 胡丰林, 陆瑞利. 尼日利亚螫毛果(Cnestis ferruginca)中一种具有清除自由基的活性化合物的分离鉴定[J]. 植物科学学报, 2002, 20(4): 311-314.
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HU Feng-Lin, LU Rui-Li. Isolation and Identification of a Free Radical Scavenger from Cnestis ferruginca[J]. Plant Science Journal, 2002, 20(4): 311-314.
Citation: HU Feng-Lin, LU Rui-Li. Isolation and Identification of a Free Radical Scavenger from Cnestis ferruginca[J]. Plant Science Journal, 2002, 20(4): 311-314.
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尼日利亚螫毛果(Cnestis ferruginca)中一种具有清除自由基的活性化合物的分离鉴定

Isolation and Identification of a Free Radical Scavenger from Cnestis ferruginca

    摘要
  • 摘要: 用二苯基苦基苯肼自由基薄层实验法(DPPH-TLC-ASSAY),从尼日利亚螫毛果(Cnestisferruginca)植物的甲醇提取物中发现了一种具有很强的清除自由基活性的物质;用柱色谱及反相制备柱色谱(RP-LPPLC),制备出了该活性物质的纯品;用质谱(HRESI-MS)、核磁共振氢谱(1H)、碳谱(13C、DEPT)、紫外光谱及化学方法对该物质进行了结构鉴定,鉴定结果为对苯酚基-6-O-反式咖啡酸基-β-D-吡喃葡萄糖甙.

     

    Abstract: By means of 1,1-dipheny-2-picylhydrazyl (DPPH) TLC-assay, a strong free radical scavenger was detected from a MeOH extract of the leaves of Cnestis ferruginca. The free radical scavenger was isolated by reversed-phase preparative chromatography and liquid chromatography, and identificated as p-hydroxyphenyl-6-O-trans-caffeoyl-β-D-glucopyranoside by means of HR ESI-MS, NMR (1H, 13C,DEPT), UVand chamical methods.The bioactivities of this compound were also discussed.

     

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