野生稻愈伤组织的超低温保存和冻后再生植株的形成

CRYOPRESERVATION AND FORMATION OF REGENERATIVE PLANTLETS OF CALLUS FROM WILD RICE

摘要: 对不同基因组的野生稻愈伤组织进行了超低温保存的研究,主要结果如下:①野生稻愈伤组织经过预培养→预处理→冰冻降温→液氮保存→快速解冻的超低温保存,冻后细胞存活率最高可达87.9%.②10%DMSO+8%葡萄糖为最佳冰冻保护剂。降温程序为0℃(这里有图片19960309-247-1.GIF)-40℃,60min→液氮(LN).③普通野生稻、宽叶野生稻、疣粒野生稻获得了冻后再生植株。④疣粒野生稻解冻后形成旺盛胚性愈伤组织,并通过体细胞胚胎发生途径再生出大量植株。

Abstract: The tissue culture and cryopreservation of 7 species of wild rice,were studied. Main results are as follows:1.By preculturing→pretreating→freezing →liquid nitrogen preserving→rapid thawing,the highest cell survival percentage of wild rice calli was 87. 9% 2.The best cryoprotectant wsa 10% DMSO plus 8% glucose for wild rice cryopreservation.And the optimal freezing procedure was:0℃(这里有图片19960309-247-1.GIF)-40℃,60min→liquid nitrogen.3.Regenerative plantlets were obtained after cryopreservation in Oryza rufipogon,O.latifolia and O. meyeriana. 4. Embryogenic callus formed after cryopreservation in O.meyeriana. And a large number of regenerative plantlets were obtained through the somatic embryogenesis way.