甘蓝分子连锁图的构建与品质性状的QTL定位

Construction of a High-density Molecular Linkage Map of Cabbage and QTLs Mapping of Several Quality Traits

摘要: 以两个不同生态型甘蓝(Brassica oleracea var.capitata)品种杂交得到的F2代为作图群体,用RAPD标记构建甘蓝分子连锁图。通过对520个随机引物进行筛选,236个引物在两亲本间表现多态性,多态性比例为47.7%。选取111个引物对群体进行分析,构建了一张含有135个标记位点,9个连锁群,覆盖长度为1023.7cM的分子连锁图。利用该图谱对甘蓝叶球紧实度和中心柱长两性状进行了QTL定位分析。检测到3个与叶球紧实度相关的QTL,总贡献率为62.5%;检测到4个与中心柱长相关的QTL,总贡献率为59.1%。

Abstract: A molecular linkage map of cabbage (Brassica oleracea var.capitata) was constructed with a 130 F₂ population from a cross of two cabbage cultivars using RAPD markers.The map covered a total of 1023.7 cM with an average interval 7.6 cM.On the basis of map,two agrono-(mic) traits,leaf head compactness and core length were analysized by using Mapmaker QTL1.1.Three QTLs for leaf head compactness (qLHC2,qLHC3,qLHC6) were located in 2nd,3rd and 6th linkage groups,they can explained about 62.5% of total phenotypic variation;four QTLs for core length (qCL2a,qCL2b,qCL3,qCL7) were located in 2nd,3rd and 7th linkage groups,they can explained about 59.1% of total phenotypic variation.