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罗杰, 刘凌, 胡道伟. 红豆杉细胞培养产物提取策略及其对紫杉醇的影响[J]. 植物科学学报, 2003, 21(2): 165-169.
引用本文: 罗杰, 刘凌, 胡道伟. 红豆杉细胞培养产物提取策略及其对紫杉醇的影响[J]. 植物科学学报, 2003, 21(2): 165-169.
LUO Jie, LIU Ling, HU Dao Wei. Influence of Taxol Production by Product Extraction Strategies in Cell Cultures of Taxus chinensis[J]. Plant Science Journal, 2003, 21(2): 165-169.
Citation: LUO Jie, LIU Ling, HU Dao Wei. Influence of Taxol Production by Product Extraction Strategies in Cell Cultures of Taxus chinensis[J]. Plant Science Journal, 2003, 21(2): 165-169.

红豆杉细胞培养产物提取策略及其对紫杉醇的影响

Influence of Taxol Production by Product Extraction Strategies in Cell Cultures of Taxus chinensis

  • 摘要: 研究了硫酸铈铵及原位提取对红豆杉细胞悬浮培养过程中细胞生长、紫杉醇合成及释放的影响。红豆杉细胞悬浮培养过程中培养第12d添加2mg/L硫酸铈铵能获得最大紫杉醇产量8.3mg/L,其中2.4mg/L释放到细胞外,分别为对照组的4倍及12倍。同时添加2mg/L硫酸铈铵、5%油酸(v/v)时胞外紫杉醇产量达到9mg/L,为对照组的45倍。将硫酸铈铵及原位提取与补料培养相结合,最高紫杉醇产量可达24.5mg/L,其中60%释放到胞外。

     

    Abstract: The effects of product release and in situ extraction on cell growth and taxol release and accumulation in cell suspension cultures of Taxus chinensis were investigated. Addition of 2 mg/L ammonium cerous sulphate on day 12 gave the greatest taxol production at 8.3 mg/L, among which 2.4 mg/L was released out of the cell, which was 4 and 12 times that of the control culture. Extracellular taxol production reached 9 mg/L when 2 mg/L ammonium cerous sulphate and 5% oleic acid (v/v) were added together, which was 45 times that of the control culture. The highest taxol accumulation of 24.5 mg/L with the release ratio of 60% was obtained when 2 mg/L ammonium cerous sulphate, 5% oleic acid (v/v) and feeding solution with 20 g/L sucrose were added.

     

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