ISSR Analysis on Genetic Diversity of Endanged Plant Pseudotsuga gaussenii Flous
LI Yan1，3；LU ShunBao1；LIU XiaoYan1，4；JIANG YuMei1；LI SiGuang4；ZHU Du1，2*
1.College of Life Sciences Jiangxi Normal University，Jiangxi Provincal Key Lab of Protection and Utilization of Subtropical Plant Resources， Nanchang 330022，China； 2.Yichun College of Jiangxi Province，Yichun，Jiangxi 336000，China； 3.Xinjian No. 2 High School of Jiangxi Province， Nanchang330100，China； 4.School of Life Sciences Nanchang University， Nanchang 330031，China
Abstract：Genetic diversity and genetic differentiation of Pseudotsuga gaussenii Flous were analyzed by using intersimple sequence repeat (ISSR) molecular technique.ISSR amplification was conducted with 10 primers for 76 individuals from 5 populations，and 69 polymorphic loci were detected from 76 loci，indicating high genetic variation at species level (PPB=90.79%，H=0.3361，I=0.4974) and at population level (PPB=56.58%-8.53%， H=0.2084-0.2681， I=02695-0.4076).A certain level of genetic differentiation occurred among populations (Gst=0.2366，Φst=26.88%).The program of genetic relationships among populations was constructed based on Nei’s gene distance.Two populations of Yangmingshan and Zhejiang clustered in a clade，then Anhui population and them clustered in a clade，and last Sanqingshan population clustered in a clade.In view of the genetic information available for .P.gaussenii. Flous，the number of .P.gaussenii. Flous has been dropping off.It is suggested that nature reserve should be founded in the distributing areas of P.gaussenii Flous to longtimely and effectively preserve the living space of P.gaussenii Flous.
2010, Vol. 28 
