Abstract: Activities and isozymes of POD and EST were analyzed with materials from different developmental stages of wheat original strains and their radiation mutants, and the relations betweens biological characters and isozymes were studied. The results showed that the height and the grain weight related to the POD and EST in reversed tendency respectively in certain stages in the first set of materials,while no obvious relationship in second set of materials. At different (developmental) stages, the bands of functional enzymes are different.