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李瑞芬, 孙振元, 魏建华, 王宏芝. 野牛草幼穗愈伤组织的诱导及植株再生[J]. 植物科学学报, 2004, 22(5): 449-454.
引用本文: 李瑞芬, 孙振元, 魏建华, 王宏芝. 野牛草幼穗愈伤组织的诱导及植株再生[J]. 植物科学学报, 2004, 22(5): 449-454.
LI Rui-Fen, SUN Zhen-Yuan, WEI Jian-Hua, WANG Hong-Zhi. Callus Induction from Immature Inflorescence and Plant Regeneration of Buffalograss[J]. Plant Science Journal, 2004, 22(5): 449-454.
Citation: LI Rui-Fen, SUN Zhen-Yuan, WEI Jian-Hua, WANG Hong-Zhi. Callus Induction from Immature Inflorescence and Plant Regeneration of Buffalograss[J]. Plant Science Journal, 2004, 22(5): 449-454.

野牛草幼穗愈伤组织的诱导及植株再生

Callus Induction from Immature Inflorescence and Plant Regeneration of Buffalograss

  • 摘要: 以野牛草Buchloe dactyloides(Nutt.)Engelm.幼穗为外植体,建立了愈伤组织诱导、继代培养和植株再生体系。结果表明,雌穗比雄穗难以脱分化形成愈伤组织;小于8mm雄幼穗在2mg/L2,4-D培养基上的愈伤组织诱导率为80.0%~86.8%;添加10mg/LAgNO3对愈伤组织诱导率影响不明显,但可改善愈伤组织质量。2mg/L2,4-D结合0.1mg/L6-BA的培养基有利于愈伤组织的继代培养;继代超过3次、继代间隔超过3周,愈伤组织分化能力明显下降。雄穗愈伤组织在含1.0mg/L6-BA培养基上,弱光条件下分化出芽的频率较高,达31.8%~35.0%;附加3%麦芽糖既可减轻褐化程度,又利于丛生芽的分化。分化苗在1/2MS+0.3mg/LIBA培养基上的生根率为62.5%。

     

    Abstract: Female and male immature inflorescences of buffalograss Buchloe dactyloides (Nutt.)Engelm. were used as explants to develop a tissue culture system for callus induction,subcultureand plant regeneration. The result showed that it is more difficult to dedifferentiate to form callusfrom female inflorescences than male ones. Callus was initiated in induction medium containing2 mg/L 2,4-D from less than 8 mm male inflorescence,the frequency was 80.0%-86.8 %. Theinclusion of 10 mg/L AgN03 in medium had no stimulatory effect on the frequency of callusinduction,however it could improve callus quality. The combination of 2 mg/L 2,4-D with0.1 mg/L 6-BA contributed to callus subculture. The regenerating potential of callus derivedfrom male inflorescence decreased while the calli were subcultured over 3 times with the internalof more than 3 weeks. The frequency of regenerating shoot ranged from 31.8%to 35.0%inmedium containing 1.0 mg/L 6-BA under the dim light. The addition of 3%maltose intoregeneration medium not only could alleviate browning,but also contributed to regenerate shoot.The rooting rate of shoots was 62.5%in 1/2 MS medium containing 0.3 mg/L IBA.

     

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