营养盐浓度对航天搭载盐藻生长及胞外多糖含量的影响

李加林; 张小平; 吴素珍; 郑维发

营养盐浓度对航天搭载盐藻生长及胞外多糖含量的影响

Effects of Nutrient Concentrations on the Growth and Accumulation of Exopolysacchrides of Spaceflight Dunaliella salina

摘要

摘要: 比较了培养液中不同营养盐浓度对“神舟5号”搭载盐藻(Dunaliella salina)和非搭载盐藻的生长及胞外多糖积累的影响,发现经“神舟5号”搭载后盐藻最适营养盐浓度发生了变化。非搭载盐藻培养液中最佳单因子组合为:CaCl₂200 mg/L、MgCl₂500 mg/L、KNO₃1000 mg/L、KH₂PO₄55 mg/L。搭载盐藻培养液中最佳单因子组合为:CaCl₂250 mg/L、MgCl₂500 mg/L、KNO₃1000 mg/L、KH₂PO₄15 mg/L。搭载和非搭载盐藻胞外多糖累积的最佳单因子组合相同:CaCl₂、MgCl₂、KNO₃、KH₂PO₄分别为250、2 000、500、15 mg/L,但搭载盐藻胞外多糖的分泌明显高于非搭载盐藻。

Abstract: Effects of nutrient concentrations on the growth and accumulation of exopolysacchrides of spaceflight and non-spaceflight Dunaliella salina were studied.It showed that the optimal combination in concentration of nutrients for their growth was different,because of the complex effects of several space environmental factors.The optimal combinations for the growth of non-spaceflight and spaceflight D.salina were CaCl₂ 200 mg/L,MgCl₂ 500 mg/L,KNO₃ 1000 mg/L,KH₂PO₄ 55 mg/L and CaCl₂ 250 mg/L,MgCl₂ 500 mg/L,KNO₃ 1000 mg/L,KH₂PO₄ 15 mg/L,respectively.The optimal combination for(accumulation) of exopolysacchrides of spaceflight and non-spaceflight D.salina was similar.It was CaCl₂ 250 mg/L,MgCl₂ 2000 mg/L,KNO₃ 500 mg/L,KH₂PO₄ 15 mg/L.The yield of exopolysacchrides of spaceflight D.salina was higher than that of non-spaceflight D.salina.

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