Callus Induction and Preliminary Study of Cell Suspension Culture in an Endangered Plant,Davidia involucrata
MAO Yan-Ping1,2, SU Zhi-Xian2, HU Jin-Yao2, CHEN Yan2, ZHONG Xiao-Cui2
1. College of Life Science, China West Normal University, Nanchong, Sichuan 637002, China;2. Mianyang Normal University, Sichuan Provincial Key Laboratory of Ecology and Environmental Technology, Mianyang, Sichuan 621000, China
Abstract：The hypocotyls from aseptic seeding of Davidia involucrata were studied via LSD analysis and orthogonal design.We firstly induced and screened the callus and then carried out suspension cultures.On this basis,we established the cell suspension culture system of Davidia involucrata,and determined the changes in biomass and pH.The results showed that the hormone combination of KT and 2,4-D induced callus were more suitable for cell suspension culture,and the best callus induction medium was WPM with KT 1.0 mg/L,2,4-D 0.5 mg/L and Vc 1.0 mg/L.The quantity of the original incubated callus and the concentration of Ca2+ were the main factors affecting biomass in the cell suspension culture system.The best quantity of original incubated callus was 25 g/L,and the best concentration of Ca2+ was two times the concentration of Ca2+ in the standard WPM medium.Under dark conditions,the growth change curve for the biomass suspension culture of Davidia involucrata resembled an"S"and the maximum biomass was available in 21 d.Results also showed a relationship between the change in pH and changes in biomass.
毛艳萍;苏智先;胡进耀;陈艳;钟小翠. 濒危植物珙桐愈伤组织的诱导及悬浮细胞培养初探[J]. 植物科学学报, 2010, 28(4): 510-515.
MAO Yan-Ping;SU Zhi-Xian;HU Jin-Yao;CHEN Yan;ZHONG Xiao-Cui. Callus Induction and Preliminary Study of Cell Suspension Culture in an Endangered Plant,Davidia involucrata. , 2010, 28(4): 510-515.
2010, Vol. 28 
