高级检索+
魏磊, 费震江, 丁毅. 紫稻(Oryza sativa L.)线粒体ATP合成酶 atp6 基因转录本 RNA 编辑[J]. 植物科学学报, 2010, 28(3): 251-256. DOI: 10.3724/SP.J.1142.2010.30251
引用本文: 魏磊, 费震江, 丁毅. 紫稻(Oryza sativa L.)线粒体ATP合成酶 atp6 基因转录本 RNA 编辑[J]. 植物科学学报, 2010, 28(3): 251-256. DOI: 10.3724/SP.J.1142.2010.30251
WEI Lei, FEI Zhen-Jiang. Mitochondrial RNA Editing of ATPase atp6 Gene Transcripts of Yunnan Purple Rice(Oryza sativa L.)[J]. Plant Science Journal, 2010, 28(3): 251-256. DOI: 10.3724/SP.J.1142.2010.30251
Citation: WEI Lei, FEI Zhen-Jiang. Mitochondrial RNA Editing of ATPase atp6 Gene Transcripts of Yunnan Purple Rice(Oryza sativa L.)[J]. Plant Science Journal, 2010, 28(3): 251-256. DOI: 10.3724/SP.J.1142.2010.30251

紫稻(Oryza sativa L.)线粒体ATP合成酶 atp6 基因转录本 RNA 编辑

Mitochondrial RNA Editing of ATPase atp6 Gene Transcripts of Yunnan Purple Rice(Oryza sativa L.)

  • 摘要: 紫稻(Oryza sativa L.)细胞质雄性不育系紫稻A是本实验室构建的新型细胞质雄性不育系。本研究使用PCR、RT-PCR、DNA测序等技术,得到了紫稻细胞质雄性不育水稻不育系(樱香A)及其保持系(樱香B)线粒体atp6基因转录本cDNA序列。通过与基因组序列比对发现:樱香Aatp6cDNA序列中,没有发生RNA编辑;而樱香Batp6 cDNA序列中有16个编辑位点,在樱香B cDNA序列16个编辑位点位于15个密码子中,所编码的氨基酸均发生改变:在1003位点由C替换为T,导致原来编码谷氨酰胺密码子(CAA)成为终止密码子(TAA),保证atp6 mRNA编码一个正常的ATP6多肽;而由于没有发生RNA编辑,樱香A mRNA就不能翻译成正常的多肽。研究表明,RNA编辑在合成正常的ATP6多肽的过程中具有至关重要的作用,同时也说明RNA编辑可能与细胞质雄性不育相关。

     

    Abstract: Base on the of construction of a new type of cytoplasmic male sterile line Zidao A(Oryza sativa L.),we analyzed the transcript editing of mitochondrial ATP synthase subunit 6 gene(atp6) from CMS line and its maintainer line.With PCR,RT-PCR,complete nucleotide sequences were determined for the mitochondrial atp6 gene cDNA from two lines of Purple Rice Type rice:cytoplasmic male sterile line Ying xiang A and its maintainer line Yingxiang B.The atp6 transcript of Yingxiang A had no editing site and the transcript of Ying xiang B had 16 editing sites with changes affecting 15 amino acid residues of the protein product.The editing of the atp6 transcript from Yingxiang B changed a glutamine codon(CAA) into a termination codon(TAA),shortening the protein of Ying xiang B to the “standard” size.The Ying xiang A transcript,which had no editing site and a“normal” termination codon,cannot be translated to a normal protein.The results demonstrated the important role of RNA editing in the production of the functional ATP6 subunit and suggested that RNA editing may be associated with cytoplasmic male sterility.

     

/

返回文章
返回