萝卜-芥蓝杂种F1代创制及杂种鉴定
Obtaining F1 Hybrid and Hybrid Identification between Raphanus sativus L.and Brassica alboglabra Bailey
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摘要: 以萝卜为母本,以芥蓝为父本,采用人工去雄授粉的方法进行杂交,然后通过胚抢救(embryo rescue)得到F1代植株,并利用RAPD技术对杂种F1代幼苗进行了鉴定.RAPD鉴定结果表明:杂种表现出亲本的特异带或者亲本不具备的新谱带,有的还遗失了亲本的特异带或者共有带.Abstract: Using hand cross and embryo rescue,F1 plants from intergeneric Raphanus sativus L.(2n=18,RR) and Brassica alboglabra Bailey (2n=18,CC) hybrids were obtained.Random amplified polymorphic DNA (RAPD) markers were used to identify the F1 plants.The RAPD results indicated that the hybrids produced specific bands of parents or new bands absent in both parents.The F1 plants also lost some specific bands or common bands of both parents.
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