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曾艳, 苏智先, 邹利娟, 吴庆贵, 毛艳萍, 程昌凤. 强德勒红心柚离体培养与植株再生体系的建立[J]. 植物科学学报, 2010, 28(2): 218-223. DOI: 10.3724/SP.J.1142.2010.20218
引用本文: 曾艳, 苏智先, 邹利娟, 吴庆贵, 毛艳萍, 程昌凤. 强德勒红心柚离体培养与植株再生体系的建立[J]. 植物科学学报, 2010, 28(2): 218-223. DOI: 10.3724/SP.J.1142.2010.20218
ZENG Yan, SU Zhi-Xian, ZOU Li-Juan, WU Qing-Gui, MAO Yan-Ping, CHENG Chang-Feng. Establishment for Culture In Vitro and Plant Regeneration System of Citrus grandis cv.Chandler[J]. Plant Science Journal, 2010, 28(2): 218-223. DOI: 10.3724/SP.J.1142.2010.20218
Citation: ZENG Yan, SU Zhi-Xian, ZOU Li-Juan, WU Qing-Gui, MAO Yan-Ping, CHENG Chang-Feng. Establishment for Culture In Vitro and Plant Regeneration System of Citrus grandis cv.Chandler[J]. Plant Science Journal, 2010, 28(2): 218-223. DOI: 10.3724/SP.J.1142.2010.20218

强德勒红心柚离体培养与植株再生体系的建立

Establishment for Culture In Vitro and Plant Regeneration System of Citrus grandis cv.Chandler

  • 摘要: 以强德勒红心柚(Citrus grandis Osbeck cv.Chandler)种子萌发的无菌苗为材料,选取子叶、子叶节段、上胚轴、带芽的茎段进行离体培养研究.结果表明:子叶节段是诱导丛生芽的最佳外植体,诱导率100%;诱导丛生芽的最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+蔗糖30 g/L+活性炭0.4 g/L,丛生芽增殖可达11.2倍;最适生根培养基为1/2MS+NAA 0.5 mg/L,生根率达100%,移栽15 d后成活率100%.

     

    Abstract: The cotyledons,cotyledonary nodes,epicotyl segments,and stem segments with lateral buds of aseptic Citrus grandis Osbeck cv.Chandler seedings were used as the explants to study the process of tissue culture rapid reproduction.Results showed that the cotyledonary nodes were the best explants of bush-bud induction(induction rate 100%).The best medium for inducing bush-bud was MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+sucrose 30 g/L+activated carbon 0.4 g/L,the coefficient of proliferation reached 11.2.The optimum medium for shoot rooting was 1/2MS+NAA 0.5 mg/L,which resulted in a rooting rate of 100%.Fifteen days after transplanting,100% of the plantlets survived.

     

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