1. 中国科学院武汉植物园, 武汉 430074;2. Department of Crop and Soil Science, Cornell University, Ithaca, NY 14853, USA
Changes of Components in Plasma Membrane of Arabidopsis Plants Overexpressed CBF3
YAN Hai-Yan1,2, STEPONKUS P L2
1. Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan 430074, China;2. Department of Crop and Soil Science, Cornell University, Ithaca, NY 14853, USA
Abstract:The function of a cold response regulator gene TBCBF3 on membrane composition was studied. Plasma membrane from transgenic plant Arabidopsis Columbia constitutively expressing CBF3 gene and from control was extracted, membrane lipids and proteins were separated for further analysis. The results show that total lipids, total proteins and phospholipids of plasma membrane increased in constitutively expressing CBF3 transgenic plant by 227%, 190% for totoal lipids, and total proteins, by 105.05% for phophoslipids. This effect is similar to that of cold acclimation, indicates that CBF3 induce the changes of the components in plasma membrane during cold acclimation, and play an important role in this process.
严海燕;STEPONKUS P L. CBF3基因过量表达的拟南芥细胞质膜组分的变化[J]. 植物科学学报, 2004, 22(6): 529-533.
YAN Hai-Yan;STEPONKUS P L. Changes of Components in Plasma Membrane of Arabidopsis Plants Overexpressed CBF3. , 2004, 22(6): 529-533.