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康薇, 郑进, 刘凯于, 彭建新, 洪华珠. 美洲黑杨(中嘉8号)离体叶片再生研究[J]. 植物科学学报, 2006, 24(1): 83-86.
引用本文: 康薇, 郑进, 刘凯于, 彭建新, 洪华珠. 美洲黑杨(中嘉8号)离体叶片再生研究[J]. 植物科学学报, 2006, 24(1): 83-86.
KANG Wei, ZHENG Jin, LIU Kai-Yu, PENG Jian-Xin, HONG Hua-Zhu. Study on Plant Regeneration of Excised Leaf from Populus deltoids(I-63×I-69)[J]. Plant Science Journal, 2006, 24(1): 83-86.
Citation: KANG Wei, ZHENG Jin, LIU Kai-Yu, PENG Jian-Xin, HONG Hua-Zhu. Study on Plant Regeneration of Excised Leaf from Populus deltoids(I-63×I-69)[J]. Plant Science Journal, 2006, 24(1): 83-86.

美洲黑杨(中嘉8号)离体叶片再生研究

Study on Plant Regeneration of Excised Leaf from Populus deltoids(I-63×I-69)

  • 摘要: 用中嘉8号杨Populus deltoids(I-63×I-69)试管苗叶片作外植体,在添加不同生长素和细胞分裂素浓度配比的1/2(N)MS培养基上,筛选出叶片直接分化不定芽的最适培养基1/2(N)MS+0.2mg/LBA+0.02mg/L NAA+25g/L蔗糖+7g/L琼脂,不定芽分化率为85%。同时发现,一定浓度的KT对生根具有促进作用,最适生根培养基为MS+0.08mg/LKT+0.02mg/L NAA+25g/L蔗糖+8g/L琼脂,生根率为82.2%。

     

    Abstract: The leaves from aseptuly plantets of Populus deltoids(I-63×I-69) were cultured as explants on 1/2(N)MS medium containing various combination of auxin and cytokinin.The results showed that the best medium for buds differentiation directly is 1/2(N)MS+0.2 mg/L BA+0.02 mg/L NAA+25 g/L sucrose+7 g/L agar,with the bud differentiation rate of 85%. In the experiments we also found KT has the stimulation to the plants rooting of I-63×I-69,the better rooting medium is MS+0.08 mg/L KT+(0.02 mg/L) NAA+25 g/L sucrose+7 g/L agar,with the rooting rate of 82.2%.

     

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