Fertility and Cytomorphology of Anthers in New Cytoplasmic Male-Sterile Lines of Chinese Cabbage (Brassica campestris L. ssp.pekinensis)
SHI Zhan1, WAN Zheng-Jie1, XU Yue-Jin1, ZOU Rui-Chang1, HUANG Tao3, FU Ting-Dong2
1. College of Horticulture and Forestry Sciences, Key Laboratory of Horticultural Plant Biology, Ministry of Education, National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan 430070, China;2. College of Plant Science and Technology, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China;3. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Abstract：In order to determine the stage and pattern of anther abortion, anatomical observations of anthers in cytoplasmic male sterile line 6w-9605A and its maintainer line 6w-9605B in Chinese cabbage (Brassica campestris L.ssp.pekinensis) were made using paraffin sections.Simultaneously,the floral organ and fertility of 6w-9605A were observed.The results showed that the anthers of maintainer line 6w-9605B were normal and the abortion of male sterile line occurred mainly at the differentiation stage of archesporial cells,which accounted for 66.7% of anther abortion and resulted in no pollen sac differentiation.In the remaining 33.3% of abortion anthers,pollen sacs were produced but the development of microspores stopped at the late uninucleate or dinucleate stages,and the hypertrophic tapetal cells compressed the microspores and caused the sterility of pollen grains.The sterility of 6w-9605A was stable and complete (100%).
施展;万正杰;徐跃进;邹瑞昌;黄涛;傅廷栋. 大白菜新型细胞质雄性不育系6w-9605A的育性鉴定和花药败育的细胞学观察[J]. 植物科学学报, 2012, 30(1): 49-54.
SHI Zhan;WAN Zheng-Jie;XU Yue-Jin;ZOU Rui-Chang;HUANG Tao;FU Ting-Dong. Fertility and Cytomorphology of Anthers in New Cytoplasmic Male-Sterile Lines of Chinese Cabbage (Brassica campestris L. ssp.pekinensis). , 2012, 30(1): 49-54.
2012, Vol. 30 
