Abstract: To clarify the genetic relationship of 96 jute (Corchorus L.) accessions including 6 species from 13 countries,sequence-related amplified polymorphism (SRAP) molecular mar-kers were used in the present study.The results showed that: (1) Genetic distance of 96 jute germplasms exhibited a large variation from 0.0169 to 0.9667,among which the relative wild species had the largest genetic distance with other germplasms,while the C.capsularis cultivars had the least genetic distance with others.(2) All 96 germplasms were classified into 3 groups and 5 unique types at the level of L₁=0.53.(3) Each group was classified into different sub-groups at the level of L₂=0.33,which showed classification according to their origins.(4) The classification of jute germplasms based on SRAP markers did not necessarily show a relationship with morphological performance.