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党峰峰, 林金辉, 陈成聪, 陈永萍, 黄国栋, 官德义, 何水林. 过量表达辣椒CaNPR1提高烟草对青枯菌的抗性[J]. 植物科学学报, 2012, 30(5): 494-500. DOI: 10.3724/SP.J.1142.2012.50494
引用本文: 党峰峰, 林金辉, 陈成聪, 陈永萍, 黄国栋, 官德义, 何水林. 过量表达辣椒CaNPR1提高烟草对青枯菌的抗性[J]. 植物科学学报, 2012, 30(5): 494-500. DOI: 10.3724/SP.J.1142.2012.50494
DANG Feng-Feng, LIN Jin-Hui, CHEN Cheng-Cong, CHEN Yong-Ping, HUANG Guo-Dong, GUAN De-Yi, HE Shui-Lin. Overexpression of CaNPR1 Enhances Resistance to Ralstonia solanacearum Infection in Tobacco[J]. Plant Science Journal, 2012, 30(5): 494-500. DOI: 10.3724/SP.J.1142.2012.50494
Citation: DANG Feng-Feng, LIN Jin-Hui, CHEN Cheng-Cong, CHEN Yong-Ping, HUANG Guo-Dong, GUAN De-Yi, HE Shui-Lin. Overexpression of CaNPR1 Enhances Resistance to Ralstonia solanacearum Infection in Tobacco[J]. Plant Science Journal, 2012, 30(5): 494-500. DOI: 10.3724/SP.J.1142.2012.50494

过量表达辣椒CaNPR1提高烟草对青枯菌的抗性

Overexpression of CaNPR1 Enhances Resistance to Ralstonia solanacearum Infection in Tobacco

  • 摘要: 对水稻和拟南芥等模式植物的研究表明,NPR1(nonexpressor of pathogenesis-related genes 1)是依赖于SA通路的防御反应调节基因,但在辣椒和烟草等茄科作物中该蛋白的功能还鲜有报道。研究从辣椒cDNA文库中分离获得一个NPR1的类似物全长cDNA(CaNPR1),并获得了其超表达的转基因烟草T1代株系。研究结果表明,这些株系与其野生型植株没有明显表型差异,但却表现出较野生型植株更高的抗青枯菌侵染活性。同时,研究还发现CaNPR1的超表达还显著提高了防御相关基因的表达,表明NPR1在不同植物间具有较强的功能保守性。

     

    Abstract: The NPR1 (nonexpressor of pathogenesis-related genes 1) gene plays an important role in SA-depended defense response processes in model plants such as Arabidopsis and rice.However,the function and underlying mechanism of this gene in non-model plants,including pepper (Capsicum annuum) and other Solanaceae,remain poorly understood.Here,we report on the functional characterization of CaNPR1 from pepper.Our data showed that the full-length cDNA of CaNPR1 was isolated and overexpression of CaNPR1 was construed in tobacco K326.Compared to wild type K326,CaNPR1-OE lines did not exhibit any phenotypic differences,but exhibited higher resistance to Ralstonia solanacearum inoculation.In addition,this was coupled with enhanced transcript levels of defense-related marker genes,suggesting that CaNPR1 possessed higher conservative function in cross species.

     

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