Regeneration System Establishment of Disease-resistant Asparagus Variety
GAO Jian-Ming1, DAI Zhen-Zhen1, YANG Feng1, ZHANG Shi-Qing1, CHEN He-Long1, ZHENG Jin-Long2, YI Ke-Xian1,2
1. Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan 571101, China;
2. Environment and Plant Protection Institute, CATAS, Danzhou, Hainan 571737, China
Abstract：Asparagus officinalis Geland is highly resistant to stem blight and was used to study the effect of different auxins and cytokines on callus induction and multiplication of buds and rooting. Results showed that the best culture medium for callus induction was MS + 6-BA 0.3 mg/L+ 2,4-D 1.5 mg/L+Sucrose 25 g/L+Agar 7 g/L, pH 5.8. The best culture medium for callus proliferation and shoot induction was MS+NAA 0.3 mg/L+6-BA 0.5 mg/L+Sucrose 30 g/L+Agar 7 g/L, pH 5.8. The best culture medium for root induction was 1/2 MS+0.5 mg/L IBA+1 mg/L PP333+ 0.3 mg/L 6-BA+ Sucrose 30 g/L+Agar 7g/L, pH 5.8.
[1] 于继庆.芦笋栽培及加工新技术[M].北京: 中国农业出版社, 1996: 1-9.[2] 李冬华,袁瑞荣,孙燕,等.中药芦笋的实验和临床研究:芦笋抗肿瘤作用和免疫调节功能的初步试验研究[J].中国临床药理学杂志, 1989, 4(1): 32.[3] 郭兵,杨蕤.芦笋对四氯化碳性肝损伤的保护作用[J].贵阳医学院学报, 1994, 19(1): 10-12.[4] 段巧玲,石莹.芦笋对四氯化碳肝损害的保护作用[J].蚌埠医学院学报, 1996, 21(4): 217-218.[5] 孙春艳,赵伯涛,郁志芳,等.芦笋的化学成分及药理作用研究进展[J].中国野生植物资源, 2004, 23(5): 1-5.[6] 冯翠萍,常霞,卢耀环.芦笋皮对实验性高脂症大鼠血脂水平的影响[J].山西农业大学学报, 2001, 21(3): 265-267.[7] 叶晓辉,陈学荣,朱亚波,等.芦笋主要病虫害的发生及防治[J].现代农业科技, 2009(7): 153.[8] 马利平,郝变青,秦曙,乔雄梧. 芦笋茎枯病的生物防治及机理研究[J].中国生态农业学报, 2009, 17(6): 1229-1233.[9] 沈火林,赵娜,韩清霞,戴凡炜.芦笋离体快速繁殖中嫩茎增殖培养对根分化的影响[J]. 华北农学报, 2006(21): 46-49.[10] 曹岩坡,代鹏,戴素英. 芦笋试管苗生根途径优化研究[J]. 河北农业科学, 2010, 14 (1): 54-55.[11] 马凤桐,郭春惹,张巧绒,李敏侠,朱象三. 芦笋优良雄株无性系的组织培养[J]. 西北农业学报, 1994(3): 40.[12] 于继庆,马秀兰,陈桂英,李芳,李少勇.芦笋茎尖组织培养技术的研究[J].山东农业科学, 1991(4): 16-20.[13] Kei-ichiro Mishiba, Kei-ichi Tawada, Masahiro Mii. Ploidy distribution in the explant tissue and the calluses induced during the initial stage of internode segment culture of Asparagus officinalis L.[J].In Vitro Cell Dev Biol-Plant, 2006(42): 83-88.[14] 徐华凌,陈纪香.耐盐芦笋的组织培养快繁技术研究[J].北方园艺, 2009(2): 92-94.[15] Limanton-Grevet A,Jullien M. Somatic embryogenesis in Asparagus officinalis can be an in vitro selection process leading to habituated and 2,4-D dependent embryogenic lines[J],Plant Physiol Biochem, 2000, 38(7): 567-576.[16] Shigeta J I, Sato K, Tanaka S, et al. Efficient plant regeneration of asparagus by inducing normal roots from in vitro multiplied shoot explants using gellan gum and glucose[J].Plant Science, 1996, 113(5): 99-104.[17] 范双喜,解淑贞.石刁柏组织培养中根诱导的研究[J].华北农学报, 1995(10): 51-54.[18] Pontaroli A C,Camadro E L. Plant regeneration after long term callus culture in clones of Asparagus officinalis L.[J].Biocell, 2005, 29(3): 313-317.[19] 母锡金.用茎段快速繁殖石刁柏[J].植物杂志, 1986(4): 28.[20] 郭春慧,马凤桐,张巧绒,李敏侠.PP333诱导芦笋试管苗生根效果的研究[J]. 西北农业大学学报, 1993, 21(3): 36-39.[21] Chen Guangyu, Conner A J, Christey M C,et al. Culture and regeneration of protoplasts from shoots of asparagus cultures [J].Int J Plant Sci, 1997, 158(5): 543-551.[22] 范双喜,解淑贞,谷建田.石刁柏离体培养快速繁育的研究[J].北京农学院学报, 1995, 10(1): 49-53.[23] 周维燕.芦笋组织培养及育种中的应用[M].北京: 高等教育出版社, 1989, 44-49.[24] 鲁旭东,萧浪涛,刘素纯,等.2,4-D对石刁柏愈伤组织诱导的影响[J].安徽农业科学, 2006, 34(21): 5546-5548.[25] 奚锡.组织培养中不定根的分化[J].植物生理生化研究进展, 1987(5): 54-64.[26] Desjardins Y, Tiessen H,Harney P M. The effect of sucrose and ancymidol on the in vitro nodal sections of asparagus[J].Hortscince, 1987, 22(1): 131-133.
2013, Vol. 31 
