Expression Pattern of FMOGS-OX4, a Biosynthetic Gene Involved in Aliphatic Glucosinolate Side-chain Modification
ZHAO Yu1, KONG Wen-Wen2, SHA Wei1, LI Jing2
1. Molecular Genetics Laboratory, College of Life Sciences and Forestry, Qiqihar University, Qiqihar, Heilongjiang 161006, China;
2. Key Laboratory of Agricultural Functional Genes, College of Life Sciences, Northeast Agriculture University, Harbin 150030, China
Abstract：Glucosinolates(GSLs)are amino acid-derived secondary metabolites,which play an important role in biotic defense reactions in plants.The bioactivity of GSLs is closely related with its chain structure.There are five Flavin-containing Monooxygenase(FMO)enzymes,FMOGS-OX1-5,involved in GSL biosynthesis catalyzing the S-oxygenation from Methylthioalkyl GSLs to Methylsulfinylalkyl GSLs.Our previous work showed that all fmogs-ox mutants,except fmogs-ox4,presented altered GSLs phenotypes.To reveal the expression characteristics and functions in GSLs biosynthesis and chain structure modification,GFP and GUS were used as reporter genes,expression pattern of FMOGS-OX4 in different tissue was analyzed and results showed that FMOGS-OX4 was expressed mainly in the vascular tissue of the leaf,stem and silique.Under normal conditions,spatial distribution of FMOGS-OX4 did not overlap with the distribution of its substrates,which was considered as the reason why fmogs-ox4 lacked altered GSLs phenotype.
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2013, Vol. 31 
