Responses of the Antioxidant System in Nelumbo nucifera Seedlings to Arsenic Toxicity
FAN Xiang-Rong1,2,3, YIN Li-Yan1,2, LI Wei1,2, CHANG Feng-Yi1,2
1. Key Laboratory of Aquatic Botany and Watershed Ecology, Chinese Academy of Sciences, Wuhan 430074, China;
2. Laboratory of Aquatic Plant Biology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China
Abstract:To study the antioxidant system of Nelumbo nucifera seedlings in response to arsenic stress, compared the influence of two valences of inorganic arsenic, As (Ⅲ) and As (V), on soluble protein content, malondialdehyde(MDA)and the antioxidant system. Results showed that: MDA and soluble protein content of N. nucifera seedlings increased and then decreased with increasing concentration; MDA and protein content were more sensitive to As(Ⅲ)than to As(V)treatment. The Superoxide Dismutase(SOD)was the most sensitive to arsenic treatment; when the concentration of As (Ⅲ)was 2.5 μmol/L and As(V)was 100 μmol/L, the activity of SOD increased significantly. When the concentration of As(Ⅲ) was 10 μmol/L, the activity of Peroxidase(POD)increased significantly. The Catalase(CAT)was more sensitive to As(V). From the experimental results, with the increase in arsenic concentration, the enzyme activities of SOD, CAT and POD increased, which can be used to eliminate reactive oxygen species(ROS) and maintain cell metabolism stability. This study provides some basic data for further study of N. nucifera physiology and growth change, cultivation and transplants with arsenic stress.
樊香绒, 尹黎燕, 李伟, 常锋毅. 中国莲(Nelumbo nucifera)幼苗抗氧化系统对砷胁迫的响应[J]. 植物科学学报, 2013, 31(6): 570-575.
FAN Xiang-Rong, YIN Li-Yan, LI Wei, CHANG Feng-Yi. Responses of the Antioxidant System in Nelumbo nucifera Seedlings to Arsenic Toxicity. , 2013, 31(6): 570-575.
[1] 洪雪花,张婉静,张爱,刘贵明,杨良,李作生.环境砷的存在状态、生物转化和修复研究进展[J].云南农业大学学报, 2011, 26(4):567-571.[2] Shri M,Kumar S,Chakrabarty D,et al.Effect of arsenic on growth,oxidative stress,and antioxidant system in rice seedlings [J].Ecotoxicol Environ Saf, 2009, 72:1102-1110.[3] LI C X.Effects of arsenic on seed germination and physiological activities of wheat seedlings [J].J Environ Sci, 2007, 19:725-732.[4] Rai S,Wahile A,Mukherjee k.Antioxidant activity of Nelumbo nucifera(sacred lotus) seeds [J].J Ethnopharmacol, 2006, 104:322-327.[5] Bradford A,Handy R D,Readman J W,et al.Impact of silver nanoparticle contamination on the genetic diversity of natural bacterial assemblages in estuarine sediments [J].Environ Sci Technol, 2009, 43(12):4530-4536.[6] 李合生.植物生理生化实验原理与技术:面向 21世纪课程教材[M].北京:高等教育出版社, 2000.[7] Cakmak I,Marschner H.Magnesium-deficiency and high light-intensity enhance activities of superoxide-dismutase,ascorbate peroxidase,and glutathione-reductase in bean-leaves [J].Plant Physiol, 1992, 98(4):1222-1227.[8] Giannopolitis C N,Ries S K.Superoxide dismutases.1.Occurrence in higher-plants [J].Plant Physiol, 1977, 59(2):309-314.[9] Xing W,Huang W,Liu G.Effect of excess iron and copper on physiology of aquatic plant Spirodela polyrrhiza (L.) Schleid [J]. Environ Toxicol, 2010, 25(2):103-112.[10] 张继飞,陈青云,吴名.重金属镉对粗梗水蕨生长及叶片中蛋白质含量的影响 [J].江汉大学学报:自然科学版, 2011, 39(4):92-104.[11] Wang H F,Zhong X H,Shi W Y,et al.Study of malondialdehyde (MDA) content,superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in chickens infected with avian infectious bronchitis virus [J].Af J Biotechno, 2011, 10(45):9213-9217.[12] 张义贤,张丽萍.重金属对大麦幼苗膜脂过氧化及脯氨酸和可溶性糖含量的影响 [J].农业环境科学学报, 2006, 25(4):857-860.[13] Blokhina O,Fagerstedt K V.Reactive oxygen species and nitric oxide in plant mitochondria:origin and redundant regulatory systems [J]. Physiol Plant, 2010, 138(4):447-462.[14] Jaspers P,Kangasjarvi J.Reactive oxygen species in abiotic stress signaling [J].Physiol Plant, 2010, 138(4):405-413.[15] Swanson S,Gilroy S.ROS in plant development [J].Physiol Plant, 2010, 138(4):384-392.[16] Verma S,Dubey R S.Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants [J].Plant Sci, 2003, 164(4):645-655.[17] Xing W,Li D,Liu G.Antioxidative responses of Elodea nuttallii (Planch.) H.St.John to short-term iron exposure [J].Plant Physiol Biochem, 2010, 48(10-11):873-478.[18] Sinha S,Saxena R.Effect of iron on lipid peroxidation,and enzymatic and non-enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L.[J].Chemosphere, 2006, 62(8):1340-1350.[19] 黄辉,李升,郭娇丽.镉胁迫对玉米幼苗抗氧化系统及光合作用的影响 [J].农业环境科学学报, 2010,29(2):211-215.[20] 于方明,刘可慧,刘华.镉污染对水稻不同生育期抗氧化系统的影响 [J].生态环境学报, 2012,21 (1):88-93.