Cloning and Expression Analysis of the SmGGPPS3 Gene from Salvia miltiorrhiza
HUA Wen-Ping1,2, SONG Shuang-Hong1, ZHI Yuan1, WANG Zhe-Zhi1
1. Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710062, China;
2. Department of Life Sciences and Technology, Shaanxi Xueqian Normal University, Xi'an 710100, China
Abstract：Geranylgeranyl pyrophosphate synthase(GGPPS) is an important target site for regulating diterpenoid biosynthesis in plant cells. Now,a novel geranylgeranyl pyrophosphate synthase gene (SmGGPPS3) was cloned from the medicinal plant Salvia miltiorrhiza. The full-length SmGGPPS3 consists of 2908 nucleotides,including a 931-bp intron and one 960-bp ORF encoding region. The deduced protein of SmGGPPS3 showed more than 67% similarity with GGPPSs from Ricinus communis,rubber and Arabidopsis.Quantitative RT-PCR analysis revealed that SmGGPPS3 was significantly expressed in different development stages and different tissues examined,and could be induced by methyl jasmonate(MeJA) and pathogens.Furthermore,the genetic complementation expression of SmGGPPS3 in Escherichia coli revealed that SmGGPPS3 encoded a functional GGPP synthase.
化文平, 宋双红, 智媛, 王喆之. 丹参SmGGPPS3基因的克隆及表达分析[J]. 植物科学学报, 2014, 32(1): 50-57.
HUA Wen-Ping, SONG Shuang-Hong, ZHI Yuan, WANG Zhe-Zhi. Cloning and Expression Analysis of the SmGGPPS3 Gene from Salvia miltiorrhiza. , 2014, 32(1): 50-57.
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2014, Vol. 32 
