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席沁, 赵廷宁, 张成梁. 若干种子处理方法对偃松种子生活力的影响[J]. 植物科学学报, 2014, 32(4): 427-433. DOI: 10.3724/SP.J.1142.2014.40427
引用本文: 席沁, 赵廷宁, 张成梁. 若干种子处理方法对偃松种子生活力的影响[J]. 植物科学学报, 2014, 32(4): 427-433. DOI: 10.3724/SP.J.1142.2014.40427
XI Qin, ZHAO Ting-Ning, ZHANG Cheng-Liang. Influence of Treatments on Pinus pumila Seed Viability[J]. Plant Science Journal, 2014, 32(4): 427-433. DOI: 10.3724/SP.J.1142.2014.40427
Citation: XI Qin, ZHAO Ting-Ning, ZHANG Cheng-Liang. Influence of Treatments on Pinus pumila Seed Viability[J]. Plant Science Journal, 2014, 32(4): 427-433. DOI: 10.3724/SP.J.1142.2014.40427

若干种子处理方法对偃松种子生活力的影响

Influence of Treatments on Pinus pumila Seed Viability

  • 摘要: 为了提高偃松种子生活力,采用水浸、NaOH溶液浸泡、PEG6000(PEG,聚乙二醇)溶液浸泡、低温冷冻、吸湿/回干5种方法对偃松种子进行处理,研究不同处理中各因素(如溶液浓度、处理时间与温度)对偃松种子生活力的影响,并采用靛蓝染色法测定偃松种子的生活力。结果表明:10%PEG6000溶液、初始温度60℃、浸泡36 h时,偃松种子生活力最强;水浸、低温处理和吸湿/回干处理方法也能提高偃松种子生活力;然而4% ~ 12% NaOH溶液浸泡处理会导致偃松种子生活力下降。因此可将PEG6000溶液处理种子的方法应用于偃松种子萌发及人工种苗培育的实践中,使偃松资源得到更好的可持续开发与利用。

     

    Abstract: In order to increase Pinus pumila seed viability, five treatments, namely, water soak, NaOH solution soak, PEG6000 (PEG, polyethylene glycol) solution soak, low-temperature, absorption of water and desiccation treatments, were used to explore factors that affect the viability of Pinus pumila seeds. Groups of each factor were tested under different concentrations, durations and temperatures, with indigo dyeing applied to measure seed viabilities. Results showed that the PEG6000 solution soak at 10% concentration, initial temperature of 60℃, and 36 h of soaking showed the most improved seed viability. Water soaking also enhanced the viability of the Pinus pumila seeds, as well as cryogenics and absorption of water and desiccation treatments. NaOH solution with concentrations of 4%-12% lowered seed viability. Testing revealed that the PEG6000 treatment was the most effective method for improving the viability of Pinus pumila, which can be applied to the cultivation and better utilization of Pinus pumila.

     

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