The activation and accumulation of pathogenesis-related protein(PR)10 play predominant roles in the defense response against pathogens. Based on a Juglans sigillata expressed sequence tag (EST) encoding PR10, gene-specific primers were designed and used to amplify the full-length cDNA of a novel PR10 gene by rapid amplification of cDNA ends (RACE). This gene was named as JsPR10-1. JsPR10-1 was 776 bp in length with an open reading frame (ORF) of 483 bp, a 5'-untranslated region (UTR) of 74 bp, and a 3'-UTR of 219 bp, and the ORF encoded a protein with 160 amino acid residues. There was an intron of 124 bp in the genomic sequence of JsPR10-1. Homology analysis indicated that JsPR10-1 was homologous with PR10s from Quercus suber,Fagus sylvatica and Castanea sativa; moreover, JsPR10-1 clustered together with the PR10s from dicots in the phylogenetic tree. qRT-PCR analysis showed that the expression levels of JsPR10-1 were induced by four different plant signaling molecules, including salicylic acid, jasmonic acid, ethylene, and H2O2. In addition, after inoculation with Colletotrichum gloeosporioides, JsPR10-1 was sharply up-regulated with the highest expression level at 8 h. The JsPR10-1 gene was involved in the defense response against C. gloeosporioides. This experiment lays a theoretical foundation for revealing the mechanism of resistance in J. sigillata.
何华, 陈朝银, 韩青, 张南南, 葛锋, 刘迪秋. 漾濞大泡核桃病程相关蛋白10基因JsPR10-1的克隆与表达分析[J]. 植物科学学报, 2014, 32(6): 612-619.
HE Hua, CHEN Chao-Yin, HAN Qing, ZHANG Nan-Nan, GE Feng, LIU Di-Qiu. Cloning and Expression Analysis of a Pathogenesis-related Protein 10 Gene from Juglans sigillata. , 2014, 32(6): 612-619.
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2014, Vol. 32 
