Abstract: DGAT2, a rate-limiting enzyme of the TAG biosynthesis pathway, was coded by the acyltransferase supergene family. Genome-wide analysis revealed 73 acyltransferase supergene family members and five DGAT2s in the genomes of Ricinus communis L., Arabidopsis thaliana Heynh., Populus trichocarpa Torr. & A. Gray. and Manihot esculenta Crantz. based on the whole genome database Phytozome. Analysis of the physical and chemical properties and transmembrane domains indicated that DGAT2s were hydrophobic transmembrane proteins, and DGAT2s of M.esculenta differed from the others due to their single transmembrane domain and wide chloroplast membrane distribution. We concluded that functional differentiation of M.esculenta DGAT2s may exist and DGAT2s may be involved in the anti-stress pathways in M.esculenta.