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蔡君龙, 卢金清, 黎强, 郭胜男, 戴艺. 不同产地丁香挥发性成分分析[J]. 植物科学学报, 2015, 33(2): 251-258. DOI: 10.11913/PSJ.2095-0837.2015.20251
引用本文: 蔡君龙, 卢金清, 黎强, 郭胜男, 戴艺. 不同产地丁香挥发性成分分析[J]. 植物科学学报, 2015, 33(2): 251-258. DOI: 10.11913/PSJ.2095-0837.2015.20251
CAI Jun-Long, LU Jin-Qing, LI Qiang, GUO Sheng-Nan, DAI Yi. Analysis on Volatile Components of Caryophylli Flos from Different Habitats[J]. Plant Science Journal, 2015, 33(2): 251-258. DOI: 10.11913/PSJ.2095-0837.2015.20251
Citation: CAI Jun-Long, LU Jin-Qing, LI Qiang, GUO Sheng-Nan, DAI Yi. Analysis on Volatile Components of Caryophylli Flos from Different Habitats[J]. Plant Science Journal, 2015, 33(2): 251-258. DOI: 10.11913/PSJ.2095-0837.2015.20251

不同产地丁香挥发性成分分析

Analysis on Volatile Components of Caryophylli Flos from Different Habitats

  • 摘要: 采用顶空固相微萃取-气相色谱-质谱联用技术对产自国内外4个地区的丁香(Eugenia caryophyllata Thunb.)挥发性成分进行了检测,并以峰面积归一化法计算了各组分的相对含量。结果显示,从4个产地13批次丁香中共鉴定出挥发性成分72种(匹配度均高于75),其中,主要成分含量排名前两位的均为丁香酚、(-)-α-芹子烯,排名第三位的挥发性成分分别为:顺式-α-没药烯(马达加斯加塔马塔夫,平均为3.75%)、罗勒烯(中国广东,平均为4.21%)、亚麻三烯(中国广西,平均为3.74%)、丁香烯(印度尼西亚爪哇岛,平均为3.60%)。表明同一产地的丁香挥发性成分具有一定的相似性,不同产地丁香挥发性成分也有一定的差异,这对丁香产地的鉴别具有重要意义。主成分分析和聚类分析的结果既可以将4个产地的丁香很好地区分开来,又能反映出它们之间的亲缘关系。本研究采用HS-SPME-GC-MS方法检测不同产地丁香挥发性成分并结合主成分分析(PCA)及聚类分析法,能有效区分4个产地的丁香,该方法可作为丁香产地的鉴别方法,也为进一步比较不同产地丁香挥发性成分的差异及质量控制提供了新思路。

     

    Abstract: We analyzed the volatile chemical components of Caryophylli Flos from different habitats using headspace solid phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and roughly quantified by peak area normalization. In total, 72 peaks were identified from 13 batches of Caryophylli Flos. Among the volatile chemical components, Eugenol and (-)-α-Selinene accounted for the first two main components, with third-ranked components of the four habitats being cis-α-bisabolene (Madagascar Tamatave, average 3.75%), Ocimene (Guangdong Province, China, average 4.21%), Santolinatriene (Guangxi Province, China, average 3.74%), Humulene (Indonesia Java, average 3.60%). These results suggest that the volatile components of Caryophylli Flos differed in different regions, but showed similarities within the same region. Using principal component analysis (PCA) and cluster analysis, results showed that the four habitat samples of Caryophylli Flos were not only easily distinguished, but also reflected a genetic relationship between them. HS-SPME-GC-MS combined with stoichiometry (PCA and cluster analysis) was able to distinguish the four origins of Caryophylli Flos. This study provided a new approach for the comparison and quality assessment of Caryophylli Flos from different habitats.

     

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