Abstract: The chromosomal locations of crossahility genes of Chinese Spring and relationship between the crossability genes and Tai-gu dominant male sterile gene Ta₁ were studied by using crosses between Chinese Spring-Cheyenne disomic substitution lines, nullitetra lines of homoeologous group 5 of Chinese Spring、Chinese Spring、Cheyenne、Ta₁ Chinese Spring and rye cultivar Lanzhou in environment of Beijing. For the first time, the results indicated that there were some crossability genes (kr) with rye in chromosome 2B、6D、7B in Chinese Spring, except crossability genes in chromosome 1A、5B、5A、5D、7D. The results also indicated that there was no interference between gene Ta₁ and crossability genes (kr). So they can independentlly express when they are in a plant. In the environment of Beijing, the kr genes express easily in Chinese Spring.