蓝花丹开花生物学观测及花粉储存研究

doi:10.11913/PSJ.2095-0837.2017.60874

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摘要

对四川引种栽培的蓝花丹（Plumbago auriculata Lam.）花粉与柱头形态、花部特征及开花物候进行了系统观测，并对其花粉的采集时间与储存温度进行了初步研究。结果显示：（1）两种类型花粉和柱头形态均具有较大差异，短花柱型（S型）花粉的极轴长P值和赤道轴长E值均显著大于长花柱型（L型）花粉，L型柱头则明显长于S型，且两种花粉的纹饰和柱头的瘤状突起物形态也明显不同；（2）蓝花丹具有较高的开花同步性指数（为0.89），但相对开花强度不高，属于中等强度；（3）蓝花丹两种花型在花蕾半开放时采收花粉，其活力最高，L型花粉活力可达85.24%±4.22%，S型可达87.74%±2.95%；（4）L型与S型花粉分别于25℃干燥0.5 h和1 h后再低温储存，其活力保持更好；（5）干燥后的花粉在-86℃条件下保存效果最好，储存30 d后L型花粉活力高达66.51%±0.85%、S型达69.07%±1.57%。本研究表明蓝花丹二型植株在生殖资源分配上存在明显差异，这种差异一方面导致花粉大小和形态及其所需干燥时间的不同，另一方面导致二型花柱头表现出明显不同的特征，这些差异性的结构是否参与了蓝花丹自交不亲和反应的识别过程，还有待进一步研究。此外，较低的开花强度会造成虫媒传粉困难，这可能是蓝花丹自然结实率极低的重要因素之一。

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	李文骥
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	雷霆
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	吴佩纹
	赵志惠

关键词 ：蓝花丹, 开花生物学, 花粉活力, 花粉存储

Abstract：

Pollen and stigma morphologies, floral characteristics, and flowering phenologies of introduced and cultivated Plumbago auriculata Lam. in Sichuan were systematically observed, with the collection period and storage temperature of pollen also examined. Results showed:(1) There were obvious differences between pollen and stigma morphology; P (polar axis length) and E (equatorial axis length) values of S-morph pollen were significantly higher than that of L-morph pollen; the stigma of the L-morph pollen was significantly longer than that of the S-morph pollen; and the exine ornamentation and stigma stromae of the two morphs of pollen were significantly different. (2) P. auriculata had a high flowering synchrony index of 0.89, but its relative flowering intensity was at a mid-level. (3) When the two morphs of pollen were harvested during the half-flowering period, viability reached the highest levels. The L-morph pollen viability reached 85.24% ±4.22% and S-morph pollen viability reached 87.74% ±2.95%. (4) L-morph and S-morph pollen maintained better viability when stored at low temperatures after drying at a constant temperature of 25℃ for 0.5 h and 1 h, respectively. (5) Dried pollen was best stored at -86℃, and after 30 d of storage the L-morph pollen viability was 66.51% ±0.85% and S-morph pollen viability was 69.07% ±1.57%. In summary, there were significant differences in the reproductive resource allocation between the two morph plants of P. auriculata, with differences in the size, morphology, and drying time of pollen, and significantly different characteristics in the stigma. Whether these different structures participate in the self-incompatibility reaction of P. auriculata remains to be further studied. In addition, low flowering intensity can cause insect pollination difficulties, which may be an important factor in the extremely low natural seed setting rate.

Key words： Plumbago auriculata Flowering biological characteristics Pollen vitality Pollen preservation

收稿日期: 2017-04-28

ZTFLH:

Q944.58

基金资助:

四川省“十三五”育种攻关项目（2016NY20038）。

通讯作者: 高素萍 E-mail: gao_suping@yahoo.com

作者简介: 李文骥(1990-),男,硕士研究生,主要从事园林植物生理研究(E-mail:liwenjilwj@126.com)。

引用本文:

李文骥, 高素萍, 雷霆, 张硕, 吴佩纹, 赵志惠. 蓝花丹开花生物学观测及花粉储存研究[J]. 植物科学学报, 2017, 35(6): 874-883. Li Wen-Ji, Gao Su-Ping, Lei Ting, Zhang Shuo, Wu Pei-Wen, Zhao Zhi-Hui. Flowering biological characteristics and pollen preservation of Plumbago auriculata. Plant Science Journal, 2017, 35(6): 874-883.

链接本文:

http://www.whzwxyj.cn/CN/10.11913/PSJ.2095-0837.2017.60874 或 http://www.whzwxyj.cn/CN/Y2017/V35/I6/874

[1] 王意成. 蓝雪花[J]. 花木盆景(花卉园艺),2015(11):44. Wang YC. Plumbago auriculata[J]. Flower Plant & Penjing, 2015(11):44.
[2] Crouch IJ, Finnie JF, Staden JV. Studies on the isolation of plumbagin from in vitro and in vivo grown Drosera species[J]. Plant Cell Tiss Org, 1990, 21(1):79-82.
[3] Moumita Gangopadhyay, Saikat Dewanjee, Dipjyoti Chakraborty. Role of exogenous phytohormones on growth and plumbagin accumulation in Plumbago indica hairy roots and conservation of elite root clones via synthetic seeds[J]. Ind Crop Prod, 2011, 33(2):445-450.
[4] Panichayupakaranant P, Tewtrakul S. Plumbagin production by root cultures of Plumbago rosea[J]. Electron J Biotechn, 2002, 5(3):228-232.
[5] 曾颖,邓燕明,姚敏元,彭健. 白花丹的药理研究及在眼科中的应用[J]. 中国药房, 1997, 8(4):161-162. Zeng Y, Deng YM, Yao MY, Pen J. The pharmacological strdy of plumbagin and its use in eye diseases[J]. China Pharmacy, 1997, 8(4):161-162.
[6] 张倩睿,梅之南,杨光忠,肖玉秀. 白花丹化学成分的研究[J]. 中药材, 2007, 30(5):558-560. Zhang QR, Mei ZN, Yang GZ, Xiao YX. Chemical consti-tuents from aerial parts of Plumbago zeylanica Linn.[J]. Journal of Chinese Medicinal Materials, 2007, 30(5):558-560.
[7] 秦鹤兰. 园林新秀蓝雪花[J]. 中国花卉园艺, 2010(24):24. Qin HL. A new horticulture cultivar Plumbago auriculata[J]. China Flowers & Horticulture, 2010(24):24.
[8] Ferrero V, Vega CD, Stafford GI, Staden JV, Johnson SD. Heterostyly and pollinators in Plumbago auriculata (Plumbaginaceae)[J]. S Afr J Bot, 2009, 75(14):778-784.
[9] Brys R, Jacquemyn H. Disruption of the distylous syndrome in Primula veris[J]. Ann Bot-London, 2015, 115(1):27-39.
[10] 张硕,高素萍. 蓝花丹的花部形态二态性及自交不亲和特性[J]. 广西植物,2014,34(6):747-753. Zhang S, Gao SP. Distyly and selfing incompatibility of Plumbago auriculata[J]. Guihaia, 2014, 34(6):747-753.
[11] 李小远,马玉磊,刘阳,唐婉, 潘会堂, 张启翔. 小报春不同授粉组合亲和性研究[J]. 西北植物学报, 2012, 32(12):2426-2431. Li XY, Ma YL, Liu Y, Tang W, Pan HT, Zhang QX. Compatibility of different pollination combinations in Primula forbesii[J]. Acta Botanica Boreali-Occidentalia, 2012, 32(12):2426-2431.
[12] Pickering CM. Variation in flowering parameters within and among five species of Australian alpine Ranunculus[J]. Aust J Bot, 1995, 43(1):103-112.
[13] 肖宜安,何平,李晓红. 濒危植物长柄双花木开花物候与生殖特性[J]. 生态学报,2004, 24(1):14-21. Xiao YA, He P, Li XP. The flowering phenology and reproductive features of the endangered plant Disanthus cercidifolius var. longipes H. T. Chang (Hamamelidaceae)[J]. Acta Ecologica,2004, 24(1):14-21.
[14] Chen ML. Comparative reproductive biology of Primula merrilliana Schltr. and P. cicutariifolia Pax.[J]. Plant Syst Evol, 2009, 278(1-2):23-32.
[15] 曹丽敏,夏念和,曹明,李湘龙. 中国无患子科的花粉形态及其系统学意义[J]. 植物科学学报, 2016, 34(6):821-833. Cao LM, Xia NH, Cao M, Li XL. Pollen morphology and its systematic significance in Sapindaceae from China[J]. Plant Sclence Journal, 2016, 34(6):821-833.
[16] 倪穗,李纪元,田敏,高捍东. 红山茶组植物花粉外壁纹饰特征及其演化关系[J].南京林业大学学报:自然科学版, 2007, 31(4):16-20. Ni S, Li JY, Tian M, Gao HD. Pollen exine sculpture of Sect. Camellia in genus Camellia and its taxonomic significance[J]. Journal of Nanjing Forestry University:Natural Science Edition, 2007, 31(4):16-20.
[17] 王宇霖. 落叶果树种类学[M]. 北京:农业出版社, 1988.
[18] 耿兴敏,黄蓓丽,罗凤霞,吴祝华, 裘建宇. 唐菖蒲花粉低温保存效果分析[J]. 南京林业大学学报:自然科学版,2011, 25(4):7-12. Geng XM, Huang BL, Luo FX, Wu ZH, Qiu JY. A study on cryopreservation of Gladiolus pollens[J]. Journal of Nanjing Forestry University:Natural Science Edition,2011, 25(4):7-12.
[19] 王钦丽,卢龙斗,吴小琴,陈祖铿,林金星. 花粉的保存及其生活力测定[J]. 植物学通报,2002, 19(3):365-373. Wang QL,Lu LD,Wu XQ,Chen ZK,Lin JX. Pollen preservation and its viability test[J]. Chinses Bulletin of Botany, 2002, 19(3):365-373.
[20] 董易,马晓红,沈强,顾俊杰, 史益敏. 荷兰水仙花粉萌发和花粉贮藏性[J]. 上海交通大学学报:农业科学版, 2012, 30(5):24-29. Dong Y, Ma XH, Shen Q, Gu JJ, Shi YM. Study on pollen germination and preservation of Narcissus pseudonarcissus[J]. Journal of Shanghai Jiaotong University:Agricultural Science, 2012, 30(5):24-29.
[21] Bhat ZA, Dhillon WS, Shafi RHS, Rather JA. Influence of storage temperature on viability and in vitro germination capacity of pear (Pyrus spp.) pollen[J]. J Agr Sci, 2012, 4(11):128-135.
[22] 李要民,陈良碧. 不同温湿条件下贮藏的3种禾本科植物花粉活力变化[J].植物生理学通讯,1998, 34(1):35-37. Li YM, Chen LB. Vigor change of several grasses pollen stored in different temperature and humidity conditions[J]. Plant Physiology Communications, 1998, 34(1):35-37.